Human TGFBR2 / Tgf Beta Receptor Ii / TGF beta R2 ELISA Kit PicoKine®

About this product

Human TGFBR2 / Tgf Beta Receptor Ii / TGF beta R2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human TGFBR2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1307

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

TGFBR2 (transforming growth factor, beta receptor II (70/80kDa) ), also known as TGF-beta receptor type-2, TGFR-2, TGF-beta type II receptor, Transforming growth factor-beta receptor type II (TGF-beta receptor type II, TbetaR-II), is a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. A TGFBR2 cDNA encoding a deduced 565-amino acid protein with a calculated molecular mass of approximately 60 kD in length. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan syndrome, Loeys-Deitz aortic aneurysm syndrome, Osler-Weber-Rendu syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different informs have been characterized. TGFBR2 may be a target of the EWS-FLI1 fusion protein found in Ewing sarcoma and related peripheral primitive neuroectodermal tumors.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 33 36 34 1.22 3.5% Sample 2 179 177 172 160 172 7.38 4.2% Sample 3 421 443 453 438 438 11.58 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 33 36 34 1.22 3.5% Sample 1 33 34 33 36 34 1.22 3.5% Sample 2 179 177 172 160 172 7.38 4.2% Sample 2 179 177 172 160 172 7.38 4.2% Sample 3 421 443 453 438 438 11.58 2.6% Sample 3 421 443 453 438 438 11.58 2.6%

Synonyms

TGF-beta receptor type-2; TGFR-2;2.7.11.30; TGF-beta type II receptor; Transforming growth factor-beta receptor type II; TGF-beta receptor type II; TbetaR-II; TGFBR2

Gene Name

TGFBR2

Gene ID

7048

UniProt

P37173

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I24-D159

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Nuclear Signaling, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction, Signaling Pathway, Signaling Pathways, Stem Cells, Surface Molecules, TGF Beta

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 179 421 30 166 392 Standard deviation 2.34 7.7%3 18.1 2.31 8.3 21.16 CV (%) 7.1% 4.6% 4.3% 7.7% 5% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 179 421 30 166 392 Standard deviation 2.34 7.7%3 18.1 2.31 8.3 21.16 CV (%) 7.1% 4.6% 4.3% 7.7% 5% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 179 421 30 166 392 Mean (pg/ml) 33 179 421 30 166 392 Standard deviation 2.34 7.7%3 18.1 2.31 8.3 21.16 Standard deviation 2.34 7.7%3 18.1 2.31 8.3 21.16 CV (%) 7.1% 4.6% 4.3% 7.7% 5% 5.4% CV (%) 7.1% 4.6% 4.3% 7.7% 5% 5.4%

Sensitivity

<10 pg/mL

Function

Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non- promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non- canonical, SMAD-independent TGF-beta signaling pathways. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

64568 MW

Specificity

Natural and recombinant human TGFBR2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

TGF-beta receptor type-2

Subcellular Location

Cell membrane ; Single-pass type I membrane protein .

Sequence Similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.

Protein Name

TGF-beta receptor type-2

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.017 0.096 0.185 0.391 0.725 1.204 1.78 1.916 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.017 0.096 0.185 0.391 0.725 1.204 1.78 1.916 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.017 0.096 0.185 0.391 0.725 1.204 1.78 1.916 O.D. 0.017 0.096 0.185 0.391 0.725 1.204 1.78 1.916

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.