- Catalog number
- EK1315
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Complement receptor type 2 (CR2), also known as C3DR or CD21, is a protein that in humans is encoded by the CR2 gene. It is mapped to 1q32.2. CR2 is involved in the complement system. It binds to iC3b (inactive derivative of C3b), C3dg, or C3d. CR2 is the membrane protein on B lymphocytes to which the Epstein-Barr virus (EBV) binds during infection of these cells. CR2, together with CD19, CD81, and CD225, forms the B-cell coreceptor complex, which lowers the activation threshold of the B-cell antigen receptor. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9) .
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 232 297 280 232 260 14.91 5.7% Sample 2 751/td> 712 1038 677 795 29.56 3.7% Sample 3 3696 2975 3237 4820 3682 241.17 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 232 297 280 232 260 14.91 5.7% Sample 1 232 297 280 232 260 14.91 5.7% Sample 2 751/td> 712 1038 677 795 29.56 3.7% Sample 2 751/td> 712 1038 677 795 29.56 3.7% Sample 3 3696 2975 3237 4820 3682 241.17 6.6% Sample 3 3696 2975 3237 4820 3682 241.17 6.6%
- Synonyms
- Complement receptor type 2; Cr2; Complement C3d receptor; Epstein-Barr virus receptor; EBV receptor; CD21; CR2; C3DR
- Gene Name
- CR2
- Gene ID
- 1380
- UniProt
- P20023
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: Ile21-71
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, B Cells, Cardiovascular, Human Protein, Immunology, Innate Immunity, Microbiology, Protein, T Cells
- Standard
- 50 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL -10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 232 751 3696 297 941 3985 Standard deviation 5.01 51.14 90.55 22.87 40.46 107.60 CV (%) 2.2% 6.8% 2.5% 7.7% 4.3% 2.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 232 751 3696 297 941 3985 Standard deviation 5.01 51.14 90.55 22.87 40.46 107.60 CV (%) 2.2% 6.8% 2.5% 7.7% 4.3% 2.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 232 751 3696 297 941 3985 Mean (pg/ml) 232 751 3696 297 941 3985 Standard deviation 5.01 51.14 90.55 22.87 40.46 107.60 Standard deviation 5.01 51.14 90.55 22.87 40.46 107.60 CV (%) 2.2% 6.8% 2.5% 7.7% 4.3% 2.7% CV (%) 2.2% 6.8% 2.5% 7.7% 4.3% 2.7%
- Sensitivity
- <75 pg/mL
- Function
- Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 112916 MW
- Specificity
- Natural and recombinant human CD21
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Complement receptor type 2
- Subcellular Location
- Membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Belongs to the receptors of complement activation (RCA) family.
- Protein Name
- Complement receptor type 2
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.032 0.067 0.128 0.279 0.538 1.045 1.895 2.28 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.032 0.067 0.128 0.279 0.538 1.045 1.895 2.28 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.032 0.067 0.128 0.279 0.538 1.045 1.895 2.28 O.D. 0.032 0.067 0.128 0.279 0.538 1.045 1.895 2.28
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
