Human CRP/C Reactive Protein ELISA Kit PicoKine®

About this product

Human CRP/C Reactive Protein ELISA Kit PicoKine® (96 Tests) . Quantitate Human CRP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1316

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 593 514 598 593 574 34.98 6% Sample 2 1488 1753 1588 1771 1650 117.62 7.1% Sample 3 8142 7585 8200 7898 7956 242.45 3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 593 514 598 593 574 34.98 6% Sample 1 593 514 598 593 574 34.98 6% Sample 2 1488 1753 1588 1771 1650 117.62 7.1% Sample 2 1488 1753 1588 1771 1650 117.62 7.1% Sample 3 8142 7585 8200 7898 7956 242.45 3% Sample 3 8142 7585 8200 7898 7956 242.45 3%

Synonyms

C-reactive protein; C-reactive protein (1-205) ; CRP; PTX1

Gene Name

CRP

Gene ID

1401

UniProt

P02741

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: F17-P224

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Found in plasma.

Applications

ELISA

Field of Research

Epigenetics and Nuclear Signaling, Metabolism, Immunology, Apoptosis, Atherosclerosis, Cardiovascular, Chromatin Binding Proteins, DNA/RNA Binding, Innate Immunity, Macrophage/Inflammation, Vascular Inflammation, Complement

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 593 1488 8142 565 1551 8792 Standard deviation 36.17 62.49 643.21 38.42 77.99 861.61 CV (%) 6.1% 4.2% 7.9% 6.8% 4.9% 9.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 593 1488 8142 565 1551 8792 Standard deviation 36.17 62.49 643.21 38.42 77.99 861.61 CV (%) 6.1% 4.2% 7.9% 6.8% 4.9% 9.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 593 1488 8142 565 1551 8792 Mean (pg/ml) 593 1488 8142 565 1551 8792 Standard deviation 36.17 62.49 643.21 38.42 77.99 861.61 Standard deviation 36.17 62.49 643.21 38.42 77.99 861.61 CV (%) 6.1% 4.2% 7.9% 6.8% 4.9% 9.8% CV (%) 6.1% 4.2% 7.9% 6.8% 4.9% 9.8%

Sensitivity

<10 pg/mL

Function

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

25039 MW

Specificity

Natural and recombinant human CRP

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-reactive protein

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the pentaxin family.

Protein Name

C-reactive protein

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.031 0.089 0.156 0.339 0.644 1.077 1.676 2.044 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.031 0.089 0.156 0.339 0.644 1.077 1.676 2.044 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.031 0.089 0.156 0.339 0.644 1.077 1.676 2.044 O.D. 0.031 0.089 0.156 0.339 0.644 1.077 1.676 2.044

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.