- Catalog number
- EK0785
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- IL17RB, also known as CRL4, is a protein that in humans is encoded by the IL17RB gene. It is located on 3p21.1. IL17RB is produced by activated T cells exhibiting proinflammatory activities. The protein encoded by this gene is a cytokine receptor. This receptor specifically binds to IL17B and IL17E (IL25), but does not bind to IL17 (A) or IL17C. This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. And it may play a role in controlling the growth and/or differentiation of hematopoietic cells.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 303 331 321 324 319 10.32 3.2% Sample 2 1191 1271 1261 1276 1249 34.34 2.7% Sample 3 5795 5828 6134 6129 5971 160.43 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 303 331 321 324 319 10.32 3.2% Sample 1 303 331 321 324 319 10.32 3.2% Sample 2 1191 1271 1261 1276 1249 34.34 2.7% Sample 2 1191 1271 1261 1276 1249 34.34 2.7% Sample 3 5795 5828 6134 6129 5971 160.43 2.6% Sample 3 5795 5828 6134 6129 5971 160.43 2.6%
- Synonyms
- Interleukin-17 receptor B; IL-17 receptor B; IL-17RB; Cytokine receptor-like 4; IL-17 receptor homolog 1; IL-17Rh1; IL17Rh1; Interleukin-17B receptor; IL-17B receptor; IL17RB; CRL4, EVI27, IL17BR; UNQ2501/PRO19612
- Gene Name
- IL17RB
- Gene ID
- 55540
- UniProt
- Q9NRM6
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: R18-K286
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed in several endocrine tissues, mostly in fetal and adult liver, kidney, pancreas, testis, colon, brain and small intestine; not detected in peripheral blood leukocytes, lymphoid organs, and most cell lines.
- Applications
- ELISA
- Field of Research
- Cancer, Cell Biology, Cell Cycle, Cell Differentiation, Cytokines, Immunology, Innate Immunity, Interleukins, Macrophage/Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 303 1191 57321 321 1173 5862 Standard deviation 14.54 47.64 411.44 17.01 58.65 527.58 CV (%) 4.8% 4% 7.1% 5.3% 5% 9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 303 1191 57321 321 1173 5862 Standard deviation 14.54 47.64 411.44 17.01 58.65 527.58 CV (%) 4.8% 4% 7.1% 5.3% 5% 9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 303 1191 57321 321 1173 5862 Mean (pg/ml) 303 1191 57321 321 1173 5862 Standard deviation 14.54 47.64 411.44 17.01 58.65 527.58 Standard deviation 14.54 47.64 411.44 17.01 58.65 527.58 CV (%) 4.8% 4% 7.1% 5.3% 5% 9% CV (%) 4.8% 4% 7.1% 5.3% 5% 9%
- Sensitivity
- <10 pg/mL
- Function
- Receptor for the proinflammatory cytokines IL17B and IL17E. May play a role in controlling the growth and/or differentiation of hematopoietic cells. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 55885 MW
- Specificity
- Natural and recombinant human IL-17RB
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin-17 receptor B
- Subcellular Location
- Isoform 1: Cell membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Contains 1 SEFIR domain.
- Protein Name
- Interleukin-17 receptor B
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.066 0.1 0.16 0.299 0.568 1.193 2.153 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.066 0.1 0.16 0.299 0.568 1.193 2.153 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.066 0.1 0.16 0.299 0.568 1.193 2.153 O.D. 0.038 0.066 0.1 0.16 0.299 0.568 1.193 2.153
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
