Human IL-17C ELISA Kit PicoKine®

About this product

Human IL-17C ELISA Kit PicoKine® (96 Tests) . Quantitate Human IL17C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0789

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

IL17C, also known as CX2, is a protein that in humans is encoded by the IL17C gene. IL17C is mapped to 16q24.3. The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. IL17C is an essential autocrine cytokine regulating innate epithelial immune responses. It also plays an important role in the pathogenesis of inflammatory arthritis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 188 158 157 172 168 12.59 7.4% Sample 2 1000 1030 955 941 981 35.48 3.6% Sample 3 5861 6256 6014 5655 5946 219.44 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 188 158 157 172 168 12.59 7.4% Sample 1 188 158 157 172 168 12.59 7.4% Sample 2 1000 1030 955 941 981 35.48 3.6% Sample 2 1000 1030 955 941 981 35.48 3.6% Sample 3 5861 6256 6014 5655 5946 219.44 3.6% Sample 3 5861 6256 6014 5655 5946 219.44 3.6%

Synonyms

Interleukin-17C; IL-17C; Cytokine CX2; IL17C; UNQ561/PRO1122

Gene Name

IL17C

Gene ID

27189

UniProt

Q9P0M4

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H19-V197

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Adaptive Immunity, Autoimmune, Cytokines, Immune System Diseases, Immunology, Innate Immunity, Interleukins

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

125 pg/mL - 8,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 188 1000 5861 172 1082 6289 Standard deviation 12.4 65 304.77 11.52 88.72 377.34 CV (%) 6.6% 6.5% 5.2% 6.7% 8.2% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 188 1000 5861 172 1082 6289 Standard deviation 12.4 65 304.77 11.52 88.72 377.34 CV (%) 6.6% 6.5% 5.2% 6.7% 8.2% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 188 1000 5861 172 1082 6289 Mean (pg/ml) 188 1000 5861 172 1082 6289 Standard deviation 12.4 65 304.77 11.52 88.72 377.34 Standard deviation 12.4 65 304.77 11.52 88.72 377.34 CV (%) 6.6% 6.5% 5.2% 6.7% 8.2% 6% CV (%) 6.6% 6.5% 5.2% 6.7% 8.2% 6%

Sensitivity

<10 pg/mL

Function

Cytokine that plays a crucial role in innate immunity of the epithelium, including to intestinal bacterial pathogens, in an autocrine manner. Stimulates the production of antibacterial peptides and proinflammatory molecules for host defense by signaling through the NF-kappa-B and MAPK pathways. Acts synergically with IL22 in inducing the expression of antibacterial peptides, including S100A8, S100A9, REG3A and REG3G. Synergy is also observed with TNF and IL1B in inducing DEFB2 from keratinocytes. Depending on the type of insult, may have both protective and pathogenic properties, either by maintaining epithelial homeostasis after an inflammatory challenge or by promoting inflammatory phenotype. Enhanced IL17C/IL17RE signaling may also lead to greater susceptibility to autoimmune diseases. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

21765 MW

Specificity

Natural and recombinant human IL-17C

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-17C

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the IL-17 family.

Protein Name

Interleukin-17C

Sample Data

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.045 0.139 0.223 0.397 0.671 1.06 1.626 2.105 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.045 0.139 0.223 0.397 0.671 1.06 1.626 2.105 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.045 0.139 0.223 0.397 0.671 1.06 1.626 2.105 O.D. 0.045 0.139 0.223 0.397 0.671 1.06 1.626 2.105

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.