- Catalog number
- EK0790
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- IL17C, also known as CX2, is a protein that in humans is encoded by the IL17C gene. IL17C is mapped to 16q24.3. The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. IL17C is an essential autocrine cytokine regulating innate epithelial immune responses. It also plays an important role in the pathogenesis of inflammatory arthritis.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 150 157 168 157 158 6.44 4% Sample 2 1098 1141 972 1172 1095 76.12 6.9% Sample 3 6281 5461 5326 5304 5593 401.73 7.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 150 157 168 157 158 6.44 4% Sample 1 150 157 168 157 158 6.44 4% Sample 2 1098 1141 972 1172 1095 76.12 6.9% Sample 2 1098 1141 972 1172 1095 76.12 6.9% Sample 3 6281 5461 5326 5304 5593 401.73 7.1% Sample 3 6281 5461 5326 5304 5593 401.73 7.1%
- Synonyms
- Interleukin-17C; Il-17c; Cytokine CX2; Il17c
- Gene Name
- IL17C
- Gene ID
- 234836
- UniProt
- Q8K4C5
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: D17-Q194
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed by epithelial cells after bacterial challenge. Low expression, if any, in lymphocytes. .
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Autoimmune, Cytokines, Immune System Diseases, Immunology, Innate Immunity, Interleukins
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 125 pg/mL - 8,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 150 1098 6281 140 1153 5857 Standard deviation 11.85 68.07 414.54 13.58 85.32 447.63 CV (%) 7.9% 6.2% 6.6% 9.7% 7.4% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 150 1098 6281 140 1153 5857 Standard deviation 11.85 68.07 414.54 13.58 85.32 447.63 CV (%) 7.9% 6.2% 6.6% 9.7% 7.4% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 150 1098 6281 140 1153 5857 Mean (pg/ml) 150 1098 6281 140 1153 5857 Standard deviation 11.85 68.07 414.54 13.58 85.32 447.63 Standard deviation 11.85 68.07 414.54 13.58 85.32 447.63 CV (%) 7.9% 6.2% 6.6% 9.7% 7.4% 7.6% CV (%) 7.9% 6.2% 6.6% 9.7% 7.4% 7.6%
- Sensitivity
- <10 pg/mL
- Function
- Cytokine that plays a crucial role in innate immunity of the epithelium, including to intestinal bacterial pathogens, in an autocrine manner. Stimulates the production of antibacterial peptides and proinflammatory molecules for host defense by signaling through the NFKB and MAPK pathways. Acts synergically with IL22, TNF and IL1B in inducing antibacterial peptides. May have protective function by maintaining epithelial homeostasis after an inflammatory challenge, such as that caused in the intestine by dextran sulfate sodium in a colitis model. May also promote an inflammatory phenotype, such as skin in a psoriasis model. Enhanced IL17C/IL17RE signaling may also lead to greater susceptibility to autoimmune diseases, such as autoimmune encephalitis. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 21592 MW
- Specificity
- Natural and recombinant mouse IL-17C
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin-17C
- Subcellular Location
- Secreted .
- Sequence Similarities
- Belongs to the IL-17 family.
- Protein Name
- Interleukin-17C
- Sample Data
- Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.033 0.115 0.192 0.325 0.608 1.07 1.77 2.417 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.033 0.115 0.192 0.325 0.608 1.07 1.77 2.417 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.033 0.115 0.192 0.325 0.608 1.07 1.77 2.417 O.D. 0.033 0.115 0.192 0.325 0.608 1.07 1.77 2.417
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
