- Catalog number
- EK1350
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- IL-20, also known as Interleukin 20 or Zcyto10, is a protein that in humans is encoded by the IL20 gene. This gene is mapped to 1q32.1. IL-20 is a protein belonging to the IL-10 family of cytokines. This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 89 90 83 88 3.63 4.1% Sample 2 232 246 237 225 235 7.64 3.2% Sample 3 997 874 895 860 906 53.71 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 89 90 83 88 3.63 4.1% Sample 1 93 89 90 83 88 3.63 4.1% Sample 2 232 246 237 225 235 7.64 3.2% Sample 2 232 246 237 225 235 7.64 3.2% Sample 3 997 874 895 860 906 53.71 5.9% Sample 3 997 874 895 860 906 53.71 5.9%
- Synonyms
- Interleukin-20; IL-20; Cytokine Zcyto10; Il20; Zcyto10
- Gene Name
- IL20
- Gene ID
- 58181
- UniProt
- Q9JKV9
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: L25-E176
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cell Type Markers, Cytokines, Immunology, Innate Immunity, Interleukins
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 93 232 997 88 234 940 Standard deviation 6.41 11.6 66.79 6.86 14.5 68.62 CV (%) 6.9% 5% 6.7% 7.8% 6.2% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 93 232 997 88 234 940 Standard deviation 6.41 11.6 66.79 6.86 14.5 68.62 CV (%) 6.9% 5% 6.7% 7.8% 6.2% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 93 232 997 88 234 940 Mean (pg/ml) 93 232 997 88 234 940 Standard deviation 6.41 11.6 66.79 6.86 14.5 68.62 Standard deviation 6.41 11.6 66.79 6.86 14.5 68.62 CV (%) 6.9% 5% 6.7% 7.8% 6.2% 7.3% CV (%) 6.9% 5% 6.7% 7.8% 6.2% 7.3%
- Sensitivity
- <10 pg/mL
- Function
- Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 20098 MW
- Specificity
- Natural and recombinant mouse IL-20
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin-20
- Subcellular Location
- Secreted.
- Protein Name
- Interleukin-20
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.01 0.039 0.069 0.137 0.276 0.612 1.222 2.319 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.01 0.039 0.069 0.137 0.276 0.612 1.222 2.319 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.01 0.039 0.069 0.137 0.276 0.612 1.222 2.319 O.D. 0.01 0.039 0.069 0.137 0.276 0.612 1.222 2.319
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
