- Catalog number
- EK0793
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin-25 (IL-25), also known as interleukin-17E (IL-17E), is a protein that in humans is encoded by the IL25 gene. It is a cytokine that belongs to the IL-17 cytokine family and mapped to 14q11.2. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. It has been found that IL-25 can induce Th2-type cytokine expression and production by non-T, non-B accessory cells that express high levels of major histocompatibility complex class II and low levels of Cd11c. This cytokine also can induce NF-κB activation, and stimulate the production of IL8. What’s more, IL-25 is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 151 150 157 155 4.84 3.1% Sample 2 689 601 681 631 650 36.2 5.5% Sample 3 1026 1036 1126 1127 1078 47.88 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 151 150 157 155 4.84 3.1% Sample 1 162 151 150 157 155 4.84 3.1% Sample 2 689 601 681 631 650 36.2 5.5% Sample 2 689 601 681 631 650 36.2 5.5% Sample 3 1026 1036 1126 1127 1078 47.88 4.4% Sample 3 1026 1036 1126 1127 1078 47.88 4.4%
- Synonyms
- Interleukin-25; IL-25; Interleukin-17E; IL-17E; IL25; IL17E; UNQ3120/PRO10272
- Gene Name
- IL25
- Gene ID
- 64806
- UniProt
- Q9H293
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: Y33-G177
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.
- Applications
- ELISA
- Field of Research
- Cytokines, Immunology, Innate Immunity, Interleukins
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 689 1026 168 748 1001 Standard deviation 8.91 42.02 50.27 10.92 57.59 53.05 CV (%) 5.5% 6.1% 4.9% 6.5% 7.7% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 689 1026 168 748 1001 Standard deviation 8.91 42.02 50.27 10.92 57.59 53.05 CV (%) 5.5% 6.1% 4.9% 6.5% 7.7% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 162 689 1026 168 748 1001 Mean (pg/ml) 162 689 1026 168 748 1001 Standard deviation 8.91 42.02 50.27 10.92 57.59 53.05 Standard deviation 8.91 42.02 50.27 10.92 57.59 53.05 CV (%) 5.5% 6.1% 4.9% 6.5% 7.7% 5.3% CV (%) 5.5% 6.1% 4.9% 6.5% 7.7% 5.3%
- Sensitivity
- <10 pg/mL
- Function
- Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 20330 MW
- Specificity
- Natural and recombinant human IL-17E
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin-25
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the IL-17 family.
- Protein Name
- Interleukin-25
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.05 0.086 0.143 0.269 0.504 0.925 1.614 2.102 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.05 0.086 0.143 0.269 0.504 0.925 1.614 2.102 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.05 0.086 0.143 0.269 0.504 0.925 1.614 2.102 O.D. 0.05 0.086 0.143 0.269 0.504 0.925 1.614 2.102
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
