- Catalog number
- EK0845
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide (LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8 (IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1 (MCP1, or SCYA2) and tumor necrosis factor (TNF) ; MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules (e.g., ITGB1) and costimulatory molecules (e.g., CD40) . Trem1 is associated with DAP12 (TYROBP), a molecule frequently associated with activating receptors.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 104 89 102 94 97 6.05 6.2% Sample 2 219 224 232 232 226 5.53 2.4% Sample 3 892 782 872 830 844 42.21 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 104 89 102 94 97 6.05 6.2% Sample 1 104 89 102 94 97 6.05 6.2% Sample 2 219 224 232 232 226 5.53 2.4% Sample 2 219 224 232 232 226 5.53 2.4% Sample 3 892 782 872 830 844 42.21 5% Sample 3 892 782 872 830 844 42.21 5%
- Synonyms
- Triggering receptor expressed on myeloid cells 1; TREM-1; CD354; Trem1
- Gene Name
- TREM1
- Gene ID
- 58217
- UniProt
- Q9JKE2
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A21-S202
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Immunology, Innate Immunity, Macrophage/Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates.
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 104 219 892 113 227 835 Standard deviation 7.28 12.26 40.14 9.26 13.84 53.44 CV (%) 7% 5.6% 4.5% 8.2% 6.1% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 104 219 892 113 227 835 Standard deviation 7.28 12.26 40.14 9.26 13.84 53.44 CV (%) 7% 5.6% 4.5% 8.2% 6.1% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 104 219 892 113 227 835 Mean (pg/ml) 104 219 892 113 227 835 Standard deviation 7.28 12.26 40.14 9.26 13.84 53.44 Standard deviation 7.28 12.26 40.14 9.26 13.84 53.44 CV (%) 7% 5.6% 4.5% 8.2% 6.1% 6.4% CV (%) 7% 5.6% 4.5% 8.2% 6.1% 6.4%
- Sensitivity
- <10 pg/mL
- Function
- Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 25409 MW
- Specificity
- Natural and recombinant mouse TREM-1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Triggering receptor expressed on myeloid cells 1
- Subcellular Location
- Membrane ; Single-pass type I membrane protein .
- Protein Name
- Triggering receptor expressed on myeloid cells 1
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.032 0.086 0.126 0.216 0.401 0.755 1.37 2.384 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.032 0.086 0.126 0.216 0.401 0.755 1.37 2.384 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.032 0.086 0.126 0.216 0.401 0.755 1.37 2.384 O.D. 0.032 0.086 0.126 0.216 0.401 0.755 1.37 2.384
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
