Human SPARCL1/SPARC-like protein 1 ELISA Kit PicoKine®

About this product

Human SPARCL1/Sparc Like 1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human SPARCL1 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1309

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. SPARCL1 is highly expressed in HEV and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 81 84 89 83 3.26 3.9% Sample 2 322 287 303 283 298 15.36 5.1% Sample 3 1261 1280 1392 1332 1316 50.87 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 81 84 89 83 3.26 3.9% Sample 1 81 81 84 89 83 3.26 3.9% Sample 2 322 287 303 283 298 15.36 5.1% Sample 2 322 287 303 283 298 15.36 5.1% Sample 3 1261 1280 1392 1332 1316 50.87 3.8% Sample 3 1261 1280 1392 1332 1316 50.87 3.8%

Synonyms

SPARC-like protein 1; High endothelial venule protein; Hevin; MAST 9; SPARCL1

Gene Name

SPARCL1

Gene ID

8404

UniProt

Q14515

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I17-F664

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes.

Applications

ELISA

Field of Research

Cancer, Oncoproteins/Suppressors, Tumor Biomarkers, Tumor Suppressors

Standard

10 ng/Tube

Sample Type

Cell culture supernatants and serum.

Detection Range

46.9 pg/mL - 3,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 322 1261 78 330 1329 Standard deviation 4.45 17.71 79.44 4.75 22.11 94.35 CV (%) 5.5% 5.5% 6.7% 6.1% 6.7% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 322 1261 78 330 1329 Standard deviation 4.45 17.71 79.44 4.75 22.11 94.35 CV (%) 5.5% 5.5% 6.7% 6.1% 6.7% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 81 322 1261 78 330 1329 Mean (pg/ml) 81 322 1261 78 330 1329 Standard deviation 4.45 17.71 79.44 4.75 22.11 94.35 Standard deviation 4.45 17.71 79.44 4.75 22.11 94.35 CV (%) 5.5% 5.5% 6.7% 6.1% 6.7% 7.1% CV (%) 5.5% 5.5% 6.7% 6.1% 6.7% 7.1%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

75208 MW

Specificity

Natural and recombinant human SPARCL1

Gene Name Synonym

SPARC-like protein 1

Subcellular Location

Secreted, extracellular space, extracellular matrix .

Sequence Similarities

Belongs to the SPARC family.

Protein Name

SPARC-like protein 1

Sample Data

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.003 0.035 0.062 0.113 0.205 0.458 0.828 1.843 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.003 0.035 0.062 0.113 0.205 0.458 0.828 1.843 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.003 0.035 0.062 0.113 0.205 0.458 0.828 1.843 O.D. 0.003 0.035 0.062 0.113 0.205 0.458 0.828 1.843

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.