- Catalog number
- EK1313
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 89 87 98 86 90 4.74 5.2% Sample 2 216 235 229 206 221 11.28 5.1% Sample 3 932 935 841 848 889 44.58 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 89 87 98 86 90 4.74 5.2% Sample 1 89 87 98 86 90 4.74 5.2% Sample 2 216 235 229 206 221 11.28 5.1% Sample 2 216 235 229 206 221 11.28 5.1% Sample 3 932 935 841 848 889 44.58 5% Sample 3 932 935 841 848 889 44.58 5%
- Synonyms
- Low affinity immunoglobulin gamma Fc region receptor II; Fc gamma receptor IIB; Fc-gamma RII; Fc-gamma-RIIB; FcRII; IgG Fc receptor II beta; Lymphocyte antigen 17; Ly-17; CD32; Fcgr2; Fcgr2b, Ly-17
- Gene Name
- FCGR2B
- Gene ID
- 14130/14131
- UniProt
- P08101/P08508
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: T40-R217
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Widely expressed by cells of hemopoietic origin. The isoforms are differentially expressed. Isoform IIB1 is preferentially expressed by cells of the lymphoid lineage, isoform IIB2 by cells of the myeloid lineage, and isoform IIB3 is released by macrophages and is present in the serum. Isoform IIB1' is expressed in myeloid and lymphoid cell lines, in normal spleen cells, and in resting or LPS-activated B-cells but is not detected in mesenteric lymph node cells.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 89 216 932 94 236 885 Standard deviation 5.05 16.63 46.6 6.95 20.06 46.9 CV (%) 5% 7.7% 5% 7.4% 8.5% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 89 216 932 94 236 885 Standard deviation 5.05 16.63 46.6 6.95 20.06 46.9 CV (%) 5% 7.7% 5% 7.4% 8.5% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 89 216 932 94 236 885 Mean (pg/ml) 89 216 932 94 236 885 Standard deviation 5.05 16.63 46.6 6.95 20.06 46.9 Standard deviation 5.05 16.63 46.6 6.95 20.06 46.9 CV (%) 5% 7.7% 5% 7.4% 8.5% 5.3% CV (%) 5% 7.7% 5% 7.4% 8.5% 5.3%
- Sensitivity
- <10 pg/mL
- Function
- Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin- coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B- cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR), T-cell receptors for AG (TCR), and Fc receptors, respectively.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 36695 MW
- Specificity
- Natural and recombinant mouse Fcgr2b/Fcgr3
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Fc receptor, IgG, low affinity IIb/ III
- Subcellular Location
- Cell membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
- Protein Name
- Low affinity immunoglobulin gamma Fc region receptor II-b/III
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.061 0.098 0.13 0.205 0.364 0.664 1.373 2.439 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.061 0.098 0.13 0.205 0.364 0.664 1.373 2.439 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.061 0.098 0.13 0.205 0.364 0.664 1.373 2.439 O.D. 0.061 0.098 0.13 0.205 0.364 0.664 1.373 2.439
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
