- Catalog number
- EK1321
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Signaling lymphocytic activation molecule is a protein that in humans is encoded by the SLAMF1 gene. It belongs to the immunoglobulin gene superfamily. This gene is mapped to 1q23.3. It has found that SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes and a proportion of B cells, and is rapidly induced on naive T cells after activation. In MV-resistant cell lines, infection with clinical MV and expression of SLAM, but not CD46, caused cytopathic effects (CPE) . The expression of SLAM on activated B and T lymphocytes correlates with the pathology of MV infection in humans and monkeys, in which lymphoid organs are the chief sites of MV replication and the binding of MV to SLAM may impair the signaling functions of SLAM in lymphocyte activation and inhibit Th0/Th1 cytokine production, thereby promoting Th2 cytokine production. It has reported that antibody-mediated ligation of SLAM on thymocytes triggered a protein tyrosine phosphorylation signal in T cells in a SAP-dependent manner. This signal also involved SHIP, the adaptor molecules DOK2, DOK1, and SHC and RASGAP.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 230 211 205 246 223 16.17 7.2% Sample 2 1313 1290 1409 1294 1326 48.41 3.6% Sample 3 5541 5036 5297 5586 5365 219.47 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 230 211 205 246 223 16.17 7.2% Sample 1 230 211 205 246 223 16.17 7.2% Sample 2 1313 1290 1409 1294 1326 48.41 3.6% Sample 2 1313 1290 1409 1294 1326 48.41 3.6% Sample 3 5541 5036 5297 5586 5365 219.47 4% Sample 3 5541 5036 5297 5586 5365 219.47 4%
- Synonyms
- Signaling lymphocytic activation molecule; CDw150; IPO-3; CD150; SLAMF1; SLAM
- Gene Name
- SLAMF1
- Gene ID
- 6504
- UniProt
- Q13291
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A21-K236
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation.
- Applications
- ELISA
- Field of Research
- Cell Type Markers, Hematopoietic Progenitors, Host-Virus Interaction, Immunology, Interspecies Interaction, Microbiology, Stem Cells, Surface Molecules
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 230 1313 5541 247 1409 5429 Standard deviation 11.27 64.33 277.05 14.82 77.49 271.45 CV (%) 4.9% 4.9% 5% 6% 5.5% 5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 230 1313 5541 247 1409 5429 Standard deviation 11.27 64.33 277.05 14.82 77.49 271.45 CV (%) 4.9% 4.9% 5% 6% 5.5% 5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 230 1313 5541 247 1409 5429 Mean (pg/ml) 230 1313 5541 247 1409 5429 Standard deviation 11.27 64.33 277.05 14.82 77.49 271.45 Standard deviation 11.27 64.33 277.05 14.82 77.49 271.45 CV (%) 4.9% 4.9% 5% 6% 5.5% 5% CV (%) 4.9% 4.9% 5% 6% 5.5% 5%
- Sensitivity
- <10 pg/mL
- Function
- High-affinity self-ligand important in biional T- cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11) -dependent signal transduction operates.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 37231 MW
- Specificity
- Natural and recombinant human SLAM
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Signaling lymphocytic activation molecule
- Subcellular Location
- Cell membrane; Single-pass type I membrane protein. Present on the surface of B-cells and T-cells.
- Sequence Similarities
- Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
- Protein Name
- Signaling lymphocytic activation molecule
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.136 0.232 0.343 0.715 1.119 1.682 2.066 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.136 0.232 0.343 0.715 1.119 1.682 2.066 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.136 0.232 0.343 0.715 1.119 1.682 2.066 O.D. 0.052 0.136 0.232 0.343 0.715 1.119 1.682 2.066
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
