- Catalog number
- EK1327
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 292 307 293 277 292 10.61 3.6% Sample 2 1705 1526 1807 1787 1706 110.86 6.4% Sample 3 4269 4351 4667 4673 4490 182.33 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 292 307 293 277 292 10.61 3.6% Sample 1 292 307 293 277 292 10.61 3.6% Sample 2 1705 1526 1807 1787 1706 110.86 6.4% Sample 2 1705 1526 1807 1787 1706 110.86 6.4% Sample 3 4269 4351 4667 4673 4490 182.33 4% Sample 3 4269 4351 4667 4673 4490 182.33 4%
- Synonyms
- 30 kDa adipocyte complement-related protein ; Adiponectin, C1Q and collagen domain containing ; Protein Adipoq ; Adipoq ; Acdc ; rCG_36676
- Gene Name
- ADIPOQ
- Gene ID
- 246253
- UniProt
- Q8K3R4
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: I19-N244
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Neurology Process, Neuroscience, Obesity, Pathways and Processes, Response To Hypoxia, Signal Transduction, Stem Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 292 1705 4269 265 1626 4075 Standard deviation 16.93 126.17 170.76 19.61 133.33 167.07 CV (%) 5.8% 7.4% 4% 7.4% 8.2% 4.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 292 1705 4269 265 1626 4075 Standard deviation 16.93 126.17 170.76 19.61 133.33 167.07 CV (%) 5.8% 7.4% 4% 7.4% 8.2% 4.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 292 1705 4269 265 1626 4075 Mean (pg/ml) 292 1705 4269 265 1626 4075 Standard deviation 16.93 126.17 170.76 19.61 133.33 167.07 Standard deviation 16.93 126.17 170.76 19.61 133.33 167.07 CV (%) 5.8% 7.4% 4% 7.4% 8.2% 4.1% CV (%) 5.8% 7.4% 4% 7.4% 8.2% 4.1%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 26410 MW
- Specificity
- Natural and recombinant rat Adiponectin
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Adiponectin, C1Q and collagen domain containing
- Protein Name
- Adiponectin
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.102 0.149 0.224 0.471 0.837 1.416 1.923 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.102 0.149 0.224 0.471 0.837 1.416 1.923 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.102 0.149 0.224 0.471 0.837 1.416 1.923 O.D. 0.017 0.102 0.149 0.224 0.471 0.837 1.416 1.923
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
