- Catalog number
- EK1339
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Syndecan 1, also known as SYND1 or CD138, is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. This gene is mapped to 2p24.1. The syndecans mediate cell binding, cell signaling, cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan 1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. What’s more, Syndecan-1 is also a sponge for growth factors, with binding largely via heparan sulfate chains.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 632 571 608 604 603 21.72 3.6% Sample 2 2595 2506 2397 2686 2546 107 4.2% Sample 3 8396 8079 9304 8737 8629 453.89 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 632 571 608 604 603 21.72 3.6% Sample 1 632 571 608 604 603 21.72 3.6% Sample 2 2595 2506 2397 2686 2546 107 4.2% Sample 2 2595 2506 2397 2686 2546 107 4.2% Sample 3 8396 8079 9304 8737 8629 453.89 5.2% Sample 3 8396 8079 9304 8737 8629 453.89 5.2%
- Synonyms
- Syndecan-1; SYND1; CD138; SDC1; SDC
- Gene Name
- SDC1
- Gene ID
- 6382
- UniProt
- P18827
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q23-G254
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cancer Metabolism, Cell Adhesion Proteins, Cell Type Marker, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Hematopoietic Progenitors, Invasion/Microenvironment, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lymphoid, Membrane Proteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Neurogenesis, Neurology Process, Neuron Marker, Neuroscience, Pathways and Processes, Signal Transduction, Stem Cells, Synapse Marker
- Standard
- 20 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 312 pg/mL - 20,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 632 25676 8396 676 2617 8785 Standard deviation 26.51 171.27 545.74 31.09 196.27 588.59 CV (%) 4.1% 6.6% 6.5% 4.6% 7.5% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 632 25676 8396 676 2617 8785 Standard deviation 26.51 171.27 545.74 31.09 196.27 588.59 CV (%) 4.1% 6.6% 6.5% 4.6% 7.5% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 632 25676 8396 676 2617 8785 Mean (pg/ml) 632 25676 8396 676 2617 8785 Standard deviation 26.51 171.27 545.74 31.09 196.27 588.59 Standard deviation 26.51 171.27 545.74 31.09 196.27 588.59 CV (%) 4.1% 6.6% 6.5% 4.6% 7.5% 6.7% CV (%) 4.1% 6.6% 6.5% 4.6% 7.5% 6.7%
- Sensitivity
- <10 pg/mL
- Function
- Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 32462 MW
- Specificity
- Natural and recombinant human Syndecan-1
- Gene Name Synonym
- Syndecan-1
- Subcellular Location
- Membrane; Single-pass type I membrane protein. Secreted. Shedding of the ectodomain produces a soluble form.
- Sequence Similarities
- Belongs to the syndecan proteoglycan family.
- Protein Name
- Syndecan-1
- Sample Data
- Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.011 0.085 0.184 0.263 0.523 0.944 1.558 2.173 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.011 0.085 0.184 0.263 0.523 0.944 1.558 2.173 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.011 0.085 0.184 0.263 0.523 0.944 1.558 2.173 O.D. 0.011 0.085 0.184 0.263 0.523 0.944 1.558 2.173
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
