- Catalog number
- EK0376
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Insulin-like growth factor 1 (IGF-1), also called somatomedin C, is a protein that in humans is encoded by the IGF1 gene. It is mapped to 12q23.2. The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 215 211 191 188 201 11.88 5.9% Sample 2 1065 1091 1134 1073 1090 26.68 2.4% Sample 3 3566 3691 3369 3307 3483 153.39 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 215 211 191 188 201 11.88 5.9% Sample 1 215 211 191 188 201 11.88 5.9% Sample 2 1065 1091 1134 1073 1090 26.68 2.4% Sample 2 1065 1091 1134 1073 1090 26.68 2.4% Sample 3 3566 3691 3369 3307 3483 153.39 4.4% Sample 3 3566 3691 3369 3307 3483 153.39 4.4%
- Synonyms
- Insulin-like growth factor I; IGF-I; Mechano growth factor; MGF; Somatomedin-C; IGF1; IBP1
- Gene Name
- IGF1
- Gene ID
- 3479
- UniProt
- P05019
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with IGF-2.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: G49-A118
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 215 1065 3566 284 1154 4861 Standard deviation 8.82 45.80 164.04 14.48 68.09 325.69 CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 215 1065 3566 284 1154 4861 Standard deviation 8.82 45.80 164.04 14.48 68.09 325.69 CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 215 1065 3566 284 1154 4861 Mean (pg/ml) 215 1065 3566 284 1154 4861 Standard deviation 8.82 45.80 164.04 14.48 68.09 325.69 Standard deviation 8.82 45.80 164.04 14.48 68.09 325.69 CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7% CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7%
- Sensitivity
- <50 pg/mL
- Function
- The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 21841 MW
- Specificity
- Natural and recombinant human IGF-1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Insulin like growth factor 1
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the insulin family.
- Protein Name
- Insulin-like growth factor I
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.046 0.087 0.128 0.215 0.393 0.674 1.232 1.803 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.046 0.087 0.128 0.215 0.393 0.674 1.232 1.803 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.046 0.087 0.128 0.215 0.393 0.674 1.232 1.803 O.D. 0.046 0.087 0.128 0.215 0.393 0.674 1.232 1.803
- Activating Reagent
- Solution A: 2Solution B: 22
- Sample Activation Procedure
- Activate the sample Cell culture supernate: add activating reagent pro rata, i.e. add 20ul of Solution A into 100ul of sample, 10 min later, add 20ul of Solution B. PH 7.0-7.6. Serum, plasma (heparin, EDTA) : add activating reagent pro rata, i.e. add 20ul of Solution A into 40ul of sample, 10 min later, add 20ul of Solution B. PH 7.0-7.6. It is unnecessary to activate the recombinant IGF-1. Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
