- Catalog number
- EK0424
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells which is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4R alpha) and at least one of two known IL-13-specific binding chains. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, signal transducer and activator of transcription 6 (STAT6) . The IL13 gene is clustered with IL3, IL5, IL4, and CSF2 on 5q.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 30 29 33 31 2.06 6.6% Sample 2 190 180 201 184 188 7.91 4.2% Sample 3 438 424 460 467 447 17.16 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 30 29 33 31 2.06 6.6% Sample 1 34 30 29 33 31 2.06 6.6% Sample 2 190 180 201 184 188 7.91 4.2% Sample 2 190 180 201 184 188 7.91 4.2% Sample 3 438 424 460 467 447 17.16 3.8% Sample 3 438 424 460 467 447 17.16 3.8%
- Synonyms
- Interleukin-13; IL-13; IL13; NC30
- Gene Name
- IL13
- Gene ID
- 3596
- UniProt
- P35225
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: G21-N132
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cytokines, Immunology, Innate Immunity, Interleukins
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 190 438 32 176 440 Standard deviation 1.93 10.64 24.09 2.36 10.56 29.92 CV (%) 5.7% 5.6% 5.5% 7.4% 6% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 190 438 32 176 440 Standard deviation 1.93 10.64 24.09 2.36 10.56 29.92 CV (%) 5.7% 5.6% 5.5% 7.4% 6% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 190 438 32 176 440 Mean (pg/ml) 34 190 438 32 176 440 Standard deviation 1.93 10.64 24.09 2.36 10.56 29.92 Standard deviation 1.93 10.64 24.09 2.36 10.56 29.92 CV (%) 5.7% 5.6% 5.5% 7.4% 6% 6.8% CV (%) 5.7% 5.6% 5.5% 7.4% 6% 6.8%
- Sensitivity
- <10 pg/mL
- Function
- Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 15816 MW
- Specificity
- Natural and recombinant human IL-13
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin-13
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the IL-4/IL-13 family.
- Protein Name
- Interleukin-13
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.021 0.072 0.117 0.195 0.362 0.702 1.288 2.097 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.021 0.072 0.117 0.195 0.362 0.702 1.288 2.097 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.021 0.072 0.117 0.195 0.362 0.702 1.288 2.097 O.D. 0.021 0.072 0.117 0.195 0.362 0.702 1.288 2.097
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
