- Catalog number
- EK1171
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What’s more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1547 1291 1390 1463 1422 94.19 6.6% Sample 2 3580 3482 3689 3752 3625 103.31 2.8% Sample 3 25269 29048 29153 26765 27558 1630.37 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1547 1291 1390 1463 1422 94.19 6.6% Sample 1 1547 1291 1390 1463 1422 94.19 6.6% Sample 2 3580 3482 3689 3752 3625 103.31 2.8% Sample 2 3580 3482 3689 3752 3625 103.31 2.8% Sample 3 25269 29048 29153 26765 27558 1630.37 5.9% Sample 3 25269 29048 29153 26765 27558 1630.37 5.9%
- Synonyms
- Pulmonary surfactant-associated protein D; PSP-D; SP-D; Collectin-7; Lung surfactant protein D; SFTPD; COLEC7, PSPD, SFTP4
- Gene Name
- SFTPD
- Gene ID
- 6441
- UniProt
- P35247
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A21-F375
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes) . .
- Applications
- ELISA
- Field of Research
- Calcium Binding Proteins, Calcium Signaling, Cancer, Cancer Metabolism, Cell Biology, Defensin, Human Protein, Immunology, Innate Immunity, Macrophage/Inflammation, Metabolism, Microbiology, Oxidative Stress, Pathways and Processes, Protein, Redox Metabolism, Signal Transduction, Signaling Pathway
- Standard
- 40 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 625 pg/mL - 40,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1547 3580 25269 1591 3588 23717 Standard deviation 92.82 221.96 1693.02 111.37 279.86 1683.9 CV (%) 6% 6.2% 6.7% 7% 7.8% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1547 3580 25269 1591 3588 23717 Standard deviation 92.82 221.96 1693.02 111.37 279.86 1683.9 CV (%) 6% 6.2% 6.7% 7% 7.8% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1547 3580 25269 1591 3588 23717 Mean (pg/ml) 1547 3580 25269 1591 3588 23717 Standard deviation 92.82 221.96 1693.02 111.37 279.86 1683.9 Standard deviation 92.82 221.96 1693.02 111.37 279.86 1683.9 CV (%) 6% 6.2% 6.7% 7% 7.8% 7.1% CV (%) 6% 6.2% 6.7% 7% 7.8% 7.1%
- Sensitivity
- <20 pg/mL
- Function
- Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 37728 MW
- Specificity
- Natural and recombinant human SP-D
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Pulmonary surfactant-associated protein D
- Subcellular Location
- Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
- Sequence Similarities
- Belongs to the SFTPD family.
- Protein Name
- Pulmonary surfactant-associated protein D
- Sample Data
- Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.09 0.138 0.179 0.286 0.497 0.766 1.456 2.084 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.09 0.138 0.179 0.286 0.497 0.766 1.456 2.084 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.09 0.138 0.179 0.286 0.497 0.766 1.456 2.084 O.D. 0.09 0.138 0.179 0.286 0.497 0.766 1.456 2.084
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
