- Catalog number
- EK1356
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Syndecan-4 (SDC4) is a protein that in humans is encoded by the SDC4 gene. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. It is mapped to 20q13.12. Syndecan-4 is one of the four vertebrate syndecans and has a molecular weight of ~20 kDa. It is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. Syndecan-4 also regulates the actin cytoskeleton, cell adhesion, and cell migration. In addition, Syndecan-4 can activate protein kinase C (PKC) . The variable domain of syndecan-4 could be a site of self-association. What’s more, Syndecan-4 also binds to phosphatidylinositol (4,5) -bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 94 82 81 78 83 6.09 7.3% Sample 2 673 735 621 630 664 45.06 6.7% Sample 3 2108 2092 1869 1903 1993 107.82 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 94 82 81 78 83 6.09 7.3% Sample 1 94 82 81 78 83 6.09 7.3% Sample 2 673 735 621 630 664 45.06 6.7% Sample 2 673 735 621 630 664 45.06 6.7% Sample 3 2108 2092 1869 1903 1993 107.82 5.4% Sample 3 2108 2092 1869 1903 1993 107.82 5.4%
- Synonyms
- Syndecan-4; SYND4; Amphiglycan; Ryudocan core protein; SDC4
- Gene Name
- SDC4
- Gene ID
- 6385
- UniProt
- P31431
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E19-E145
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed in epithelial and fibroblastic cells. .
- Applications
- ELISA
- Field of Research
- Cell Adhesion Proteins, Cytoskeleton/ECM, ECM Proteins, Neurogenesis, Neurology Process, Neuroscience, Plasma Membrane, Signal Transduction, Subcellular Markers, Tags & Cell Markers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates and serum.
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 94 673 2108 102 680 1958 Standard deviation 3.85 52.49 128.58 5.4 56.44 154.68 CV (%) 4.1% 7.8% 6.1% 5.3% 8.3% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 94 673 2108 102 680 1958 Standard deviation 3.85 52.49 128.58 5.4 56.44 154.68 CV (%) 4.1% 7.8% 6.1% 5.3% 8.3% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 94 673 2108 102 680 1958 Mean (pg/ml) 94 673 2108 102 680 1958 Standard deviation 3.85 52.49 128.58 5.4 56.44 154.68 Standard deviation 3.85 52.49 128.58 5.4 56.44 154.68 CV (%) 4.1% 7.8% 6.1% 5.3% 8.3% 7.9% CV (%) 4.1% 7.8% 6.1% 5.3% 8.3% 7.9%
- Sensitivity
- <10 pg/mL
- Function
- Cell surface proteoglycan that bears heparan sulfate.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 21642 MW
- Specificity
- Natural and recombinant human Syndecan-4
- Gene Name Synonym
- Syndecan-4
- Subcellular Location
- Isoform 1: Membrane; Single-pass type I membrane protein. Secreted. Shedding of the ectodomain produces a soluble form. .
- Protein Name
- Syndecan-4
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.053 0.099 0.131 0.195 0.332 0.621 1.158 2.261 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.053 0.099 0.131 0.195 0.332 0.621 1.158 2.261 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.053 0.099 0.131 0.195 0.332 0.621 1.158 2.261 O.D. 0.053 0.099 0.131 0.195 0.332 0.621 1.158 2.261
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
