- Catalog number
- EK1357
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Biglycan (BGN) is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. It is an important structural component of articular cartilage and participates in the assembly of the chondrocyte extracellular matrix through formation of protein interactions with type VI collagen and large proteoglycan aggregates. In humans, Biglycan is encoded by the BGN gene. It is mapped to Xq28. Biglycan is believed to play a role in the mineralisation of bone, and its core protein binds to the growth factors BMP-4 and influences its bioactivity. It has been reported that the presence of biglycan is necessary for BMP-4 to exert its effects on osteoblasts. There is also evidence that biglycan can bind to TGF-beta 1. In addition, Biglycan plays an important role in cell signaling.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 190 188 215 183 194 12.38 6.3% Sample 2 939 960 955 1125 994 75.59 7.6% Sample 3 4176 4429 4400 3916 4230 206.16 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 190 188 215 183 194 12.38 6.3% Sample 1 190 188 215 183 194 12.38 6.3% Sample 2 939 960 955 1125 994 75.59 7.6% Sample 2 939 960 955 1125 994 75.59 7.6% Sample 3 4176 4429 4400 3916 4230 206.16 4.8% Sample 3 4176 4429 4400 3916 4230 206.16 4.8%
- Synonyms
- Biglycan; Bone/cartilage proteoglycan I; PG-S1; BGN; SLRR1A
- Gene Name
- BGN
- Gene ID
- 633
- UniProt
- P21810
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: D38-K368
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Found in several connective tissues, especially in articular cartilages.
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Cardiovascular, Immunology, Innate Immunity, Macrophage/Inflammation, Organelles, Subcellular Markers, T Cells, Tags & Cell Markers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 190 939 4176 179 981 4439 Standard deviation 9.12 54.46 167.04 10.2 56.89 217.51 CV (%) 4.8% 5.8% 4% 5.7% 5.8% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 190 939 4176 179 981 4439 Standard deviation 9.12 54.46 167.04 10.2 56.89 217.51 CV (%) 4.8% 5.8% 4% 5.7% 5.8% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 190 939 4176 179 981 4439 Mean (pg/ml) 190 939 4176 179 981 4439 Standard deviation 9.12 54.46 167.04 10.2 56.89 217.51 Standard deviation 9.12 54.46 167.04 10.2 56.89 217.51 CV (%) 4.8% 5.8% 4% 5.7% 5.8% 4.9% CV (%) 4.8% 5.8% 4% 5.7% 5.8% 4.9%
- Sensitivity
- <10 pg/mL
- Function
- May be involved in collagen fiber assembly. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 41654 MW
- Specificity
- Natural and recombinant human Biglycan
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Biglycan
- Subcellular Location
- Secreted, extracellular space, extracellular matrix .
- Sequence Similarities
- Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
- Protein Name
- Biglycan
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.064 0.118 0.215 0.45 0.798 1.578 2.017 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.064 0.118 0.215 0.45 0.798 1.578 2.017 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.064 0.118 0.215 0.45 0.798 1.578 2.017 O.D. 0.004 0.064 0.118 0.215 0.45 0.798 1.578 2.017
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
