- Catalog number
- EK1358
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- TFPI2, also called Tissue factor pathway inhibitor 2, is a protein that in humans is encoded by the TFPI2 gene. It is mapped to 7q21.3. This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Qualitative PCR and microarray analyses showed that expression of TFPI2 was inversely correlated with that of MIR616 in prostate cancer cell lines, primary tumors of various grades, and normal tissue.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 143 191 141 241 179 5.96 3.3% Sample 2 811 946 836 757 838 62.98 7.5% Sample 3 2856 2959 2892 3245 2988 154.78 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 143 191 141 241 179 5.96 3.3% Sample 1 143 191 141 241 179 5.96 3.3% Sample 2 811 946 836 757 838 62.98 7.5% Sample 2 811 946 836 757 838 62.98 7.5% Sample 3 2856 2959 2892 3245 2988 154.78 5.2% Sample 3 2856 2959 2892 3245 2988 154.78 5.2%
- Synonyms
- Tissue factor pathway inhibitor 2; TFPI-2; Placental protein 5; PP5; TFPI2
- Gene Name
- TFPI2
- Gene ID
- 7980
- UniProt
- P48307
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: D23-F235
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy.
- Applications
- ELISA
- Field of Research
- Cardiovascular, Cell Biology, Protease Inhibitors, Proteolysis/Ubiquitin
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 125 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 143 811 2856 190 797 3495 Standard deviation 7.56 48.5 130.52 10.30 33.16 89.47 CV (%) 5.3% 6.0% 4.6% 5.4% 4.2% 2.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 143 811 2856 190 797 3495 Standard deviation 7.56 48.5 130.52 10.30 33.16 89.47 CV (%) 5.3% 6.0% 4.6% 5.4% 4.2% 2.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 143 811 2856 190 797 3495 Mean (pg/ml) 143 811 2856 190 797 3495 Standard deviation 7.56 48.5 130.52 10.30 33.16 89.47 Standard deviation 7.56 48.5 130.52 10.30 33.16 89.47 CV (%) 5.3% 6.0% 4.6% 5.4% 4.2% 2.6% CV (%) 5.3% 6.0% 4.6% 5.4% 4.2% 2.6%
- Sensitivity
- <10 pg/mL
- Function
- May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 26934 MW
- Specificity
- Natural and recombinant human TFPI2
- Gene Name Synonym
- Tissue factor pathway inhibitor 2
- Subcellular Location
- Secreted.
- Protein Name
- Tissue factor pathway inhibitor 2
- Sample Data
- Concentration (pg/ml) 0 125 250 500 1000 2000 4000 O.D. 0.031 0.257 0.471 0.84 1.389 2.02 2.386 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 O.D. 0.031 0.257 0.471 0.84 1.389 2.02 2.386 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 O.D. 0.031 0.257 0.471 0.84 1.389 2.02 2.386 O.D. 0.031 0.257 0.471 0.84 1.389 2.02 2.386
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
