- Catalog number
- EK0792
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin 17D (IL-17D) is a member of the unique IL-17 family, which share a conserved cysteine motif. It is mapped to 13q12.11. IL-17D regulates cytokine production in endothelial cells and inhibits hemopoiesis in vitro. Purified recombinant IL-17D inhibited myeloid progenitor cell colony formation. IL-17D can enhance endothelial cell production of IL6, IL8, and GMCSF, but not other cytokines. And it is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 147 150 156 137 147 6.87 4.6% Sample 2 775 677 680 767 724 46.34 6.4% Sample 3 1330 1370 1170 1232 1275 78.93 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 147 150 156 137 147 6.87 4.6% Sample 1 147 150 156 137 147 6.87 4.6% Sample 2 775 677 680 767 724 46.34 6.4% Sample 2 775 677 680 767 724 46.34 6.4% Sample 3 1330 1370 1170 1232 1275 78.93 6.1% Sample 3 1330 1370 1170 1232 1275 78.93 6.1%
- Synonyms
- IL-17D ; Il17d
- Gene Name
- IL17D
- Gene ID
- 239114
- UniProt
- Q8K4C4
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A25-R205
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 147 775 1330 157 700 1222 Standard deviation 5.88 51.15 98.42 8.79 50.4 97.76 CV (%) 4% 6.6% 7.4% 5.6% 7.2% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 147 775 1330 157 700 1222 Standard deviation 5.88 51.15 98.42 8.79 50.4 97.76 CV (%) 4% 6.6% 7.4% 5.6% 7.2% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 147 775 1330 157 700 1222 Mean (pg/ml) 147 775 1330 157 700 1222 Standard deviation 5.88 51.15 98.42 8.79 50.4 97.76 Standard deviation 5.88 51.15 98.42 8.79 50.4 97.76 CV (%) 4% 6.6% 7.4% 5.6% 7.2% 8% CV (%) 4% 6.6% 7.4% 5.6% 7.2% 8%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 22391 MW
- Specificity
- Natural and recombinant mouse IL-17D
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin 17D
- Protein Name
- Interleukin-17D
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.142 0.197 0.316 0.586 0.984 1.446 2.096 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.142 0.197 0.316 0.586 0.984 1.446 2.096 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.142 0.197 0.316 0.586 0.984 1.446 2.096 O.D. 0.004 0.142 0.197 0.316 0.586 0.984 1.446 2.096
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
