Mouse IL-34/Interleukin-34 ELISA Kit PicoKine®

About this product

Mouse IL-34 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Il34 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1366

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-34, or IL-34 is a protein belonging to a group of cytokines called interleukins. It is mapped to 16q22.1. The discovery of IL-34 protein in the red pulp of the spleen suggests involvement in growth and development of myeloid cells, consistent with its activity on monocytes. IL-34 is a recently discovered cytokine that acts on tissue resident macrophages and Langerhans cells upon binding the receptor for CSF-1, CSF-1R. It also can promote the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor. In addition, it promotes the development, survival, and function of microglia and Langerhans cells, therefore, this cytokine may predominately function in brain and skin biology.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 85 84 80 78 81 2.86 3.5% Sample 2 457 389 454 435 433 27.17 6.2% Sample 3 1273 1355 1390 1363 1345 43.68 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 85 84 80 78 81 2.86 3.5% Sample 1 85 84 80 78 81 2.86 3.5% Sample 2 457 389 454 435 433 27.17 6.2% Sample 2 457 389 454 435 433 27.17 6.2% Sample 3 1273 1355 1390 1363 1345 43.68 3.2% Sample 3 1273 1355 1390 1363 1345 43.68 3.2%

Synonyms

Interleukin-34; IL-34; Il34

Gene Name

IL34

Gene ID

76527

UniProt

Q8R1R4

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N21-P235

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins, Macrophage/Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 85 457 1273 79 494 1320 Standard deviation 3.4 31.07 84.9 4.42 36.55 104.28 CV (%) 4% 6.8% 6.7% 5.6% 7.4% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 85 457 1273 79 494 1320 Standard deviation 3.4 31.07 84.9 4.42 36.55 104.28 CV (%) 4% 6.8% 6.7% 5.6% 7.4% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 85 457 1273 79 494 1320 Mean (pg/ml) 85 457 1273 79 494 1320 Standard deviation 3.4 31.07 84.9 4.42 36.55 104.28 Standard deviation 3.4 31.07 84.9 4.42 36.55 104.28 CV (%) 4% 6.8% 6.7% 5.6% 7.4% 7.9% CV (%) 4% 6.8% 6.7% 5.6% 7.4% 7.9%

Sensitivity

<10 pg/mL

Function

Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1 (By similarity) . .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

26764 MW

Specificity

Natural and recombinant mouse IL-34

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-34

Subcellular Location

Secreted .

Protein Name

Interleukin-34

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.049 0.127 0.204 0.378 0.657 1.038 1.587 1.963 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.049 0.127 0.204 0.378 0.657 1.038 1.587 1.963 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.049 0.127 0.204 0.378 0.657 1.038 1.587 1.963 O.D. 0.049 0.127 0.204 0.378 0.657 1.038 1.587 1.963

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.