- Catalog number
- EK1368
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Phospholipid transfer protein (PLTP), also known as lipid transfer protein II is a protein that in humans is encoded by the PLTP gene. This gene is mapped to 20q13.12. The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL) . In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1418 1318 1305 1394 1358 48.22 3.5% Sample 2 4309 4364 4776 4611 4515 188.79 4.1% Sample 3 22189 22076 22370 22574 22302 188.71 0.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1418 1318 1305 1394 1358 48.22 3.5% Sample 1 1418 1318 1305 1394 1358 48.22 3.5% Sample 2 4309 4364 4776 4611 4515 188.79 4.1% Sample 2 4309 4364 4776 4611 4515 188.79 4.1% Sample 3 22189 22076 22370 22574 22302 188.71 0.8% Sample 3 22189 22076 22370 22574 22302 188.71 0.8%
- Synonyms
- Phospholipid transfer protein; Lipid transfer protein II; Pltp
- Gene Name
- PLTP
- Gene ID
- 18830
- UniProt
- P55065
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E18-A493
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Highest in lung, adipose tissue, brain, and heart.
- Applications
- ELISA
- Field of Research
- Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Cholesterol Metabolism, Heart Disease, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Pathways and Processes, Serum Proteins, Signal Transduction
- Standard
- 50 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 0.78 ng/mL - 50 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1418 4309 22189 1296 4212 21284 Standard deviation 87.91 284.39 1020.69 99.79 320.11 1213.18 CV (%) 6.2% 6.6% 4.6% 7.7% 7.6% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1418 4309 22189 1296 4212 21284 Standard deviation 87.91 284.39 1020.69 99.79 320.11 1213.18 CV (%) 6.2% 6.6% 4.6% 7.7% 7.6% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1418 4309 22189 1296 4212 21284 Mean (pg/ml) 1418 4309 22189 1296 4212 21284 Standard deviation 87.91 284.39 1020.69 99.79 320.11 1213.18 Standard deviation 87.91 284.39 1020.69 99.79 320.11 1213.18 CV (%) 6.2% 6.6% 4.6% 7.7% 7.6% 5.7% CV (%) 6.2% 6.6% 4.6% 7.7% 7.6% 5.7%
- Sensitivity
- <20 pg/mL
- Function
- Facilitates the transfer of a spectrum of different lipid molecules, including diacylglycerol, phosphatidic acid, sphingomyelin, phosphatidylcholine, phosphatidylglycerol, cerebroside and phosphatidyl ethanolamine. Essential for the transfer of excess surface lipids from triglyceride-rich lipoproteins to HDL, thereby facilitating the formation of smaller lipoprotein remnants, contributing to the formation of LDL, and assisting in the maturation of HDL particles. PLTP also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma: an active form that can transfer PC from phospholipid vesicles to high-density lipoproteins (HDL), and an inactive form that lacks this capability (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 54453 MW
- Specificity
- Natural and recombinant mouse PLTP
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Phospholipid transfer protein
- Subcellular Location
- Secreted.
- Protein Name
- Phospholipid transfer protein
- Sample Data
- Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.006 0.046 0.093 0.187 0.345 0.743 1.247 2.004 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.006 0.046 0.093 0.187 0.345 0.743 1.247 2.004 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.006 0.046 0.093 0.187 0.345 0.743 1.247 2.004 O.D. 0.006 0.046 0.093 0.187 0.345 0.743 1.247 2.004
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
