- Catalog number
- EK1372
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1127 1186 1062 1229 1151 62.86 5.4% Sample 2 4290 4643 4014 4511 4364 238.44 5.4% Sample 3 29063 29288 30434 26599 28846 1397.6 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1127 1186 1062 1229 1151 62.86 5.4% Sample 1 1127 1186 1062 1229 1151 62.86 5.4% Sample 2 4290 4643 4014 4511 4364 238.44 5.4% Sample 2 4290 4643 4014 4511 4364 238.44 5.4% Sample 3 29063 29288 30434 26599 28846 1397.6 4.8% Sample 3 29063 29288 30434 26599 28846 1397.6 4.8%
- Synonyms
- A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM-TS 4; ADAM-TS4; ADAMTS-4;3.4.24.82; ADMP-1; Aggrecanase-1; ADAMTS4; KIAA0688; UNQ769/PRO1563
- Gene Name
- ADAMTS4
- Gene ID
- 9507
- UniProt
- O75173
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: F213-C685
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles. Isoform 2 is detected in osteoarthritic synovium. .
- Applications
- ELISA
- Field of Research
- Cancer, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction
- Standard
- 40 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 625 pg/mL - 40,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1127 4290 29063 1210 4672 29313 Standard deviation 46.2 184.47 1627.52 71.39 200.89 2081.22 CV (%) 4.1% 4.3% 5.6% 5.9% 4.3% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1127 4290 29063 1210 4672 29313 Standard deviation 46.2 184.47 1627.52 71.39 200.89 2081.22 CV (%) 4.1% 4.3% 5.6% 5.9% 4.3% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1127 4290 29063 1210 4672 29313 Mean (pg/ml) 1127 4290 29063 1210 4672 29313 Standard deviation 46.2 184.47 1627.52 71.39 200.89 2081.22 Standard deviation 46.2 184.47 1627.52 71.39 200.89 2081.22 CV (%) 4.1% 4.3% 5.6% 5.9% 4.3% 7.1% CV (%) 4.1% 4.3% 5.6% 5.9% 4.3% 7.1%
- Sensitivity
- <20 pg/mL
- Function
- Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-|-Ala-393' site.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 90197 MW
- Specificity
- Natural and recombinant human ADAMTS4
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- A disintegrin and metalloproteinase with thrombospondin motifs 4
- Subcellular Location
- Secreted, extracellular space, extracellular matrix .
- Sequence Similarities
- Contains 1 disintegrin domain.
- Protein Name
- A disintegrin and metalloproteinase with thrombospondin motifs 4
- Sample Data
- Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.064 0.16 0.222 0.308 0.522 0.921 1.448 2.465 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.064 0.16 0.222 0.308 0.522 0.921 1.448 2.465 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.064 0.16 0.222 0.308 0.522 0.921 1.448 2.465 O.D. 0.064 0.16 0.222 0.308 0.522 0.921 1.448 2.465
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
