- Catalog number
- EK0521
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- TIMP metallopeptidase inhibitor 1, also known as TIMP1, a tissue inhibitor of metalloproteinases, is a glycoprotein that is expressed from the several tissues of organisms. This protein is a member of the TIMP family. It was found to reside at Xp11.4-p11.1. This is the first growth factor found to be X-linked. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. What’s more, TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 65 74 72 73 71 3.53 4.9% Sample 2 258 273 282 277 272 8.95 3.2% Sample 3 787 883 759 760 797 50.76 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 65 74 72 73 71 3.53 4.9% Sample 1 65 74 72 73 71 3.53 4.9% Sample 2 258 273 282 277 272 8.95 3.2% Sample 2 258 273 282 277 272 8.95 3.2% Sample 3 787 883 759 760 797 50.76 6.3% Sample 3 787 883 759 760 797 50.76 6.3%
- Synonyms
- Metalloproteinase inhibitor 1; Collagenase inhibitor 16C8 fibroblast; Erythroid-potentiating activity; EPA; TPA-S1; TPA-induced protein; Tissue inhibitor of metalloproteinases 1; TIMP-1; Timp1; Timp, Timp-1
- Gene Name
- TIMP1
- Gene ID
- 21857
- UniProt
- P12032
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: C25-R205
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Found in fetal and adult tissues. Highest levels are found in bone. Also found in lung, ovary and uterus.
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Protease Inhibitors, Proteolysis/Ubiquitin, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 65 258 787 62 246 710 Standard deviation 3.44 16.51 41.71 4.21 20.41 45.44 CV (%) 5.3% 6.4% 5.3% 6.8% 8.3% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 65 258 787 62 246 710 Standard deviation 3.44 16.51 41.71 4.21 20.41 45.44 CV (%) 5.3% 6.4% 5.3% 6.8% 8.3% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 65 258 787 62 246 710 Mean (pg/ml) 65 258 787 62 246 710 Standard deviation 3.44 16.51 41.71 4.21 20.41 45.44 Standard deviation 3.44 16.51 41.71 4.21 20.41 45.44 CV (%) 5.3% 6.4% 5.3% 6.8% 8.3% 6.4% CV (%) 5.3% 6.4% 5.3% 6.8% 8.3% 6.4%
- Sensitivity
- <10 pg/mL
- Function
- Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14 (By similarity) . Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 22628 MW
- Specificity
- Natural and recombinant mouse TIMP-1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Metalloproteinase inhibitor 1
- Subcellular Location
- Secreted .
- Sequence Similarities
- Belongs to the protease inhibitor I35 (TIMP) family.
- Protein Name
- Metalloproteinase inhibitor 1
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.04 0.13 0.208 0.364 0.614 1.002 1.59 2.232 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.04 0.13 0.208 0.364 0.614 1.002 1.59 2.232 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.04 0.13 0.208 0.364 0.614 1.002 1.59 2.232 O.D. 0.04 0.13 0.208 0.364 0.614 1.002 1.59 2.232
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
