- Catalog number
- EK0805
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 479 546 475 475 493 30.21 6.1% Sample 2 2934 2910 2938 2814 2899 50.22 1.7% Sample 3 8702 9816 8527 9384 9107 519.57 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 479 546 475 475 493 30.21 6.1% Sample 1 479 546 475 475 493 30.21 6.1% Sample 2 2934 2910 2938 2814 2899 50.22 1.7% Sample 2 2934 2910 2938 2814 2899 50.22 1.7% Sample 3 8702 9816 8527 9384 9107 519.57 5.7% Sample 3 8702 9816 8527 9384 9107 519.57 5.7%
- Synonyms
- Mannose-binding protein C; MBP-C; Collectin-1; MBP1; Mannan-binding protein; Mannose-binding lectin; MBL2; COLEC1, MBL
- Gene Name
- MBL2
- Gene ID
- 4153
- UniProt
- P11226
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E21-I248
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Plasma protein produced mainly in the liver. .
- Applications
- ELISA
- Field of Research
- Complement, Immunology, Innate Immunity
- Standard
- 20 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 312 pg/mL - 20,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 479 2854 8702 458 2785 8839 Standard deviation 27.3 129.09 661.35 34.35 147.6 742.47 CV (%) 5.7% 4.4% 7.6% 7.5% 5.3% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 479 2854 8702 458 2785 8839 Standard deviation 27.3 129.09 661.35 34.35 147.6 742.47 CV (%) 5.7% 4.4% 7.6% 7.5% 5.3% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 479 2854 8702 458 2785 8839 Mean (pg/ml) 479 2854 8702 458 2785 8839 Standard deviation 27.3 129.09 661.35 34.35 147.6 742.47 Standard deviation 27.3 129.09 661.35 34.35 147.6 742.47 CV (%) 5.7% 4.4% 7.6% 7.5% 5.3% 8.4% CV (%) 5.7% 4.4% 7.6% 7.5% 5.3% 8.4%
- Sensitivity
- <10 pg/mL
- Function
- Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 26144 MW
- Specificity
- Natural and recombinant human MBP-C
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Mannose-binding protein C
- Subcellular Location
- Secreted .
- Sequence Similarities
- Contains 1 C-type lectin domain.
- Protein Name
- Mannose-binding protein C
- Sample Data
- Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.01 0.091 0.171 0.35 0.627 1.05 1.66 2.217 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.01 0.091 0.171 0.35 0.627 1.05 1.66 2.217 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.01 0.091 0.171 0.35 0.627 1.05 1.66 2.217 O.D. 0.01 0.091 0.171 0.35 0.627 1.05 1.66 2.217
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
