- Catalog number
- EK0529
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Tumor necrosis factor receptor 1 (TNFR1), also known as tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and CD120a, is a ubiquitous membrane receptor that binds tumor necrosis factor-alpha (TNF alpha) . The protein encoded by this gene is a member of the tumor necrosis factor receptor superfamily, which also contains TNFRSF1B. And this protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate the transcription factor NF-κB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 39 42 39 38 2.48 6.5% Sample 2 224 227 240 237 232 6.67 2.8% Sample 3 495 438 426 477 459 28.06 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 39 42 39 38 2.48 6.5% Sample 1 35 39 42 39 38 2.48 6.5% Sample 2 224 227 240 237 232 6.67 2.8% Sample 2 224 227 240 237 232 6.67 2.8% Sample 3 495 438 426 477 459 28.06 6.1% Sample 3 495 438 426 477 459 28.06 6.1%
- Synonyms
- Tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor 1; TNF-R1; Tumor necrosis factor receptor type I; TNF-RI; TNFR-I; p55; p60; CD120a; Tnfrsf1a; Tnfr-1, Tnfr1
- Gene Name
- Tnfrsf1a
- Gene ID
- 21937
- UniProt
- P25118
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L30-A212
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 224 437 37 204 459 Standard deviation 1.5 13.88 27.22 1.7 18%2 27.08 CV (%) 4.3% 6.2% 5.5% 4.6% 8% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 224 437 37 204 459 Standard deviation 1.5 13.88 27.22 1.7 18%2 27.08 CV (%) 4.3% 6.2% 5.5% 4.6% 8% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 224 437 37 204 459 Mean (pg/ml) 35 224 437 37 204 459 Standard deviation 1.5 13.88 27.22 1.7 18%2 27.08 Standard deviation 1.5 13.88 27.22 1.7 18%2 27.08 CV (%) 4.3% 6.2% 5.5% 4.6% 8% 5.9% CV (%) 4.3% 6.2% 5.5% 4.6% 8% 5.9%
- Sensitivity
- <10 pg/mL
- Function
- Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 50130 MW
- Specificity
- Natural and recombinant mouse TNFR1
- Gene Name Synonym
- Tumor necrosis factor receptor superfamily member 1A
- Subcellular Location
- Cell membrane; Single-pass type I membrane protein. Golgi apparatus membrane ; Single-pass type I membrane protein .
- Protein Name
- Tumor necrosis factor receptor superfamily member 1A
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.121 0.188 0.34 0.629 1.036 1.742 2.297 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.121 0.188 0.34 0.629 1.036 1.742 2.297 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.121 0.188 0.34 0.629 1.036 1.742 2.297 O.D. 0.025 0.121 0.188 0.34 0.629 1.036 1.742 2.297
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
