- Catalog number
- EK0725
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Macrophage Inflammatory Protein 2 (MIP2), also known as C-X-C motif chemokine 2 (CXCL2), is a small cytokine belonging to the CXC chemokine family. MIP2 forms homotetramers. MIP2 is produced by activated monocytes and neutrophils and expressed at sites of inflammation. MIP2 is chemotactic for polymorphonuclear leukocytes.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 35 35 36 35 0.43 1.2% Sample 2 171 176 185 176 177 5.04 2.8% Sample 3 537 483 467 489 494 26.09 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 35 35 36 35 0.43 1.2% Sample 1 35 35 35 36 35 0.43 1.2% Sample 2 171 176 185 176 177 5.04 2.8% Sample 2 171 176 185 176 177 5.04 2.8% Sample 3 537 483 467 489 494 26.09 5.2% Sample 3 537 483 467 489 494 26.09 5.2%
- Synonyms
- C-X-C motif chemokine 2; Cytokine-induced neutrophil chemoattractant 3; CINC-3; Macrophage inflammatory protein 2; MIP2; Cxcl2; Cinc3, Mip-2, Mip2, Scyb2
- Gene Name
- Cxcl2
- Gene ID
- 114105
- UniProt
- P30348
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: S32-N100
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- At least expressed in the lung and trachea.
- Applications
- ELISA
- Field of Research
- Chemokines, Immunology, Innate Immunity
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 171 537 31 178 563 Standard deviation 2.69 2.89 21.48 2.88 8.36 27.02 CV (%) 7.7% 4.6% 4% 9.3% 4.7% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 171 537 31 178 563 Standard deviation 2.69 2.89 21.48 2.88 8.36 27.02 CV (%) 7.7% 4.6% 4% 9.3% 4.7% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 171 537 31 178 563 Mean (pg/ml) 35 171 537 31 178 563 Standard deviation 2.69 2.89 21.48 2.88 8.36 27.02 Standard deviation 2.69 2.89 21.48 2.88 8.36 27.02 CV (%) 7.7% 4.6% 4% 9.3% 4.7% 4.8% CV (%) 7.7% 4.6% 4% 9.3% 4.7% 4.8%
- Sensitivity
- <10 pg/mL
- Function
- Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. Contributes to neutrophil activation during inflammation.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 10783 MW
- Specificity
- Natural and recombinant rat MIP-2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-X-C motif chemokine 2
- Subcellular Location
- Secreted.
- Protein Name
- C-X-C motif chemokine 2
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.047 0.112 0.159 0.276 0.509 0.905 1.549 2.294 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.047 0.112 0.159 0.276 0.509 0.905 1.549 2.294 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.047 0.112 0.159 0.276 0.509 0.905 1.549 2.294 O.D. 0.047 0.112 0.159 0.276 0.509 0.905 1.549 2.294
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
