- Catalog number
- EK0747
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 84 77 79 90 82 5.02 6.1% Sample 2 591 589 657 668 626 36.46 5.8% Sample 3 1510 1598 1670 1616 1598 57.55 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 84 77 79 90 82 5.02 6.1% Sample 1 84 77 79 90 82 5.02 6.1% Sample 2 591 589 657 668 626 36.46 5.8% Sample 2 591 589 657 668 626 36.46 5.8% Sample 3 1510 1598 1670 1616 1598 57.55 3.6% Sample 3 1510 1598 1670 1616 1598 57.55 3.6%
- Synonyms
- Neuroblastoma suppressor of tumorigenicity 1; N03; Zinc finger protein DAN; Nbl1; Dan, Dana
- Gene Name
- Nbl1
- Gene ID
- 17965
- UniProt
- Q61477
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A17-D178
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Growth Factors/Hormones, Oncoproteins/Suppressors, Signal Transduction, Tumor Suppressors
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 84 591 1510 86 596 1475 Standard deviation 5.54 31.91 78.52 6.7 37.54 89.97 CV (%) 6.6% 5.4% 5.2% 7.8% 6.3% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 84 591 1510 86 596 1475 Standard deviation 5.54 31.91 78.52 6.7 37.54 89.97 CV (%) 6.6% 5.4% 5.2% 7.8% 6.3% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 84 591 1510 86 596 1475 Mean (pg/ml) 84 591 1510 86 596 1475 Standard deviation 5.54 31.91 78.52 6.7 37.54 89.97 Standard deviation 5.54 31.91 78.52 6.7 37.54 89.97 CV (%) 6.6% 5.4% 5.2% 7.8% 6.3% 6.1% CV (%) 6.6% 5.4% 5.2% 7.8% 6.3% 6.1%
- Sensitivity
- <10 pg/mL
- Function
- Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 19107 MW
- Specificity
- Natural and recombinant mouse DAN
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Neuroblastoma suppressor of tumorigenicity 1
- Subcellular Location
- Secreted .
- Protein Name
- Neuroblastoma suppressor of tumorigenicity 1
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.027 0.084 0.138 0.264 0.491 0.831 1.34 2.07 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.027 0.084 0.138 0.264 0.491 0.831 1.34 2.07 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.027 0.084 0.138 0.264 0.491 0.831 1.34 2.07 O.D. 0.027 0.084 0.138 0.264 0.491 0.831 1.34 2.07
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
