- Catalog number
- EK0784
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin 17 receptor A, also known as IL17RA and CDw217 (cluster of differentiation w217), is a human gene. The IL17R gene is mapped to 22q11.22-q11.23 by radiation hybrid analysis. Interleukin 17A (IL17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The protein encoded by this gene is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Also, it is showed that mouse Il17r bound HSV-13 and mouse Il17. Recombinant soluble Il17r inhibited mitogen-induced T-cell proliferation and Il2 production.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 84 78 90 82 83 4.33 5.2% Sample 2 276 303 258 288 281 16.48 5.8% Sample 3 1012 1007 960 871 962 56.58 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 84 78 90 82 83 4.33 5.2% Sample 1 84 78 90 82 83 4.33 5.2% Sample 2 276 303 258 288 281 16.48 5.8% Sample 2 276 303 258 288 281 16.48 5.8% Sample 3 1012 1007 960 871 962 56.58 5.8% Sample 3 1012 1007 960 871 962 56.58 5.8%
- Synonyms
- Interleukin-17 receptor A; IL-17 receptor A; IL-17RA; CDw217; CD217; IL17RA; IL17R
- Gene Name
- IL17RA
- Gene ID
- 23765
- UniProt
- Q96F46
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L33-W320
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Widely expressed. .
- Applications
- ELISA
- Field of Research
- Cytokines, Immunology, Innate Immunity, Interleukins, Macrophage/Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 84 276 1012 83 250 1067 Standard deviation 6.63 19.32 72.86 7.3 19.25 87.49 CV (%) 7.9% 7% 7.2% 8.8% 7.7% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 84 276 1012 83 250 1067 Standard deviation 6.63 19.32 72.86 7.3 19.25 87.49 CV (%) 7.9% 7% 7.2% 8.8% 7.7% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 84 276 1012 83 250 1067 Mean (pg/ml) 84 276 1012 83 250 1067 Standard deviation 6.63 19.32 72.86 7.3 19.25 87.49 Standard deviation 6.63 19.32 72.86 7.3 19.25 87.49 CV (%) 7.9% 7% 7.2% 8.8% 7.7% 8.2% CV (%) 7.9% 7% 7.2% 8.8% 7.7% 8.2%
- Sensitivity
- <10 pg/mL
- Function
- Receptor for IL17A (PubMed:17911633, PubMed:9367539) . Receptor for IL17F (PubMed:19838198, PubMed:17911633) . Binds to IL17A with higher affinity than to IL17F (PubMed:17911633) . Binds IL17A and IL17F homodimers as part of a heterodimeric complex with IL17RC (PubMed:16785495) . Also binds heterodimers formed by IL17A and IL17F as part of a heterodimeric complex with IL17RC (PubMed:18684971) . Receptor for IL17C as part of a heterodimeric complex with IL17RE (PubMed:21993848) . Activation of IL17RA leads to induction of expression of inflammatory chemokines and cytokines such as CXCL1, CXCL8/IL8 and IL6 (PubMed:16785495, PubMed:17911633, PubMed:18684971) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 96122 MW
- Specificity
- Natural and recombinant human IL-17RA
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin-17 receptor A
- Subcellular Location
- Isoform 1: Cell membrane ; Single-pass type I membrane protein .
- Protein Name
- Interleukin-17 receptor A
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.078 0.129 0.222 0.4 0.671 1.198 1.982 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.078 0.129 0.222 0.4 0.671 1.198 1.982 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.078 0.129 0.222 0.4 0.671 1.198 1.982 O.D. 0.019 0.078 0.129 0.222 0.4 0.671 1.198 1.982
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
