- Catalog number
- EK0972
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Nerve growth factor receptor (NGFR) is also referred to as p75 (NTR) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. The nerve growth factor receptor gene is at human chromosome region 17q12-17q22, distal to the chromosome 17 breakpoint in acute leukemias. The neurotrophin receptor p75 (NTR), a tumor necrosis factor receptor superfamily member expressed in hepatic stellate cells after fibrotic and cirrhotic liver injury in humans, is a regulator of liver repair. In mice, depletion of p75 (NTR) exacerbated liver pathology and inhibited hepatocyte proliferation in vivo. In addition, it is showed that neurotrophins activate p75 (NTR) to induce apoptosis through the induction of the sphingomyelin (SM) cycle and increased production of ceramide. Overexpression of p75 (NTR) is also found to activate the SM pathway.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 194 175 185 176 182 7.69 4.2% Sample 2 811 686 734 797 757 50.21 6.6% Sample 3 1003 1138 1104 1038 1070 53.12 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 194 175 185 176 182 7.69 4.2% Sample 1 194 175 185 176 182 7.69 4.2% Sample 2 811 686 734 797 757 50.21 6.6% Sample 2 811 686 734 797 757 50.21 6.6% Sample 3 1003 1138 1104 1038 1070 53.12 4.9% Sample 3 1003 1138 1104 1038 1070 53.12 4.9%
- Synonyms
- Tumor necrosis factor receptor superfamily member 16; Gp80-LNGFR; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; NGF receptor; p75 ICD; CD271; NGFR; TNFRSF16
- Gene Name
- NGFR
- Gene ID
- 4804
- UniProt
- P08138
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: K29-N250
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Growth and Development, Mesenchymal Stem Cells, Neural Stem Cells, Neurology Process, Neuroscience, Stem Cells, Surface Molecules
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 194 811 1003 200 784 959 Standard deviation 11.44 46.22 52.15 12.6 46.25 53.7 CV (%) 5.2%% 5.7% 5.2% 5.9%% 5.9% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 194 811 1003 200 784 959 Standard deviation 11.44 46.22 52.15 12.6 46.25 53.7 CV (%) 5.2%% 5.7% 5.2% 5.9%% 5.9% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 194 811 1003 200 784 959 Mean (pg/ml) 194 811 1003 200 784 959 Standard deviation 11.44 46.22 52.15 12.6 46.25 53.7 Standard deviation 11.44 46.22 52.15 12.6 46.25 53.7 CV (%) 5.2%% 5.7% 5.2% 5.9%% 5.9% 5.6% CV (%) 5.2%% 5.7% 5.2% 5.9%% 5.9% 5.6%
- Sensitivity
- <10 pg/mL
- Function
- Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity) . Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 45183 MW
- Specificity
- Natural and recombinant human TNFRSF16
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Tumor necrosis factor receptor superfamily member 16
- Subcellular Location
- Membrane; Single-pass type I membrane protein.
- Protein Name
- Tumor necrosis factor receptor superfamily member 16
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.028 0.076 0.137 0.245 0.482 0.849 1.345 1.97 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.028 0.076 0.137 0.245 0.482 0.849 1.345 1.97 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.028 0.076 0.137 0.245 0.482 0.849 1.345 1.97 O.D. 0.028 0.076 0.137 0.245 0.482 0.849 1.345 1.97
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
