- Catalog number
- EK1167
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Kallikrein-12 is a protein that in humans is encoded by the KLK12 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. KLK12 is expressed primarily in salivary gland, stomach, uterus, trachea, prostate, thymus, lung, colon, brain, breast, and thyroid gland and at lower levels in testis, pancreas, small intestine, and spinal cord. The expression of KLK12 is downregulated at the mRNA level in breast cancer tissues and is upregulated by steroid hormones in breast and prostate cancer cell lines.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 208 206 217 216 211 4.81 2.2% Sample 2 1283 1297 1371 1367 1329 39.83 2.9% Sample 3 4339 4587 4086 4442 4363 182.83 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 208 206 217 216 211 4.81 2.2% Sample 1 208 206 217 216 211 4.81 2.2% Sample 2 1283 1297 1371 1367 1329 39.83 2.9% Sample 2 1283 1297 1371 1367 1329 39.83 2.9% Sample 3 4339 4587 4086 4442 4363 182.83 4.1% Sample 3 4339 4587 4086 4442 4363 182.83 4.1%
- Synonyms
- Kallikrein-12;3.4.21.-; Kallikrein-like protein 5; KLK-L5; KLK12; KLKL5; UNQ669/PRO1303
- Gene Name
- KLK12
- Gene ID
- 43849
- UniProt
- Q9UKR0
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A18-N248
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 208 1283 4339 215 1293 3970 Standard deviation 8.94 92.37 260.34 9.67 93.09 273.93 CV (%) 4.3% 7.2% 6% 4.5% 7.2% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 208 1283 4339 215 1293 3970 Standard deviation 8.94 92.37 260.34 9.67 93.09 273.93 CV (%) 4.3% 7.2% 6% 4.5% 7.2% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 208 1283 4339 215 1293 3970 Mean (pg/ml) 208 1283 4339 215 1293 3970 Standard deviation 8.94 92.37 260.34 9.67 93.09 273.93 Standard deviation 8.94 92.37 260.34 9.67 93.09 273.93 CV (%) 4.3% 7.2% 6% 4.5% 7.2% 6.9% CV (%) 4.3% 7.2% 6% 4.5% 7.2% 6.9%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 26734 MW
- Specificity
- Natural and recombinant human KLK12
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Kallikrein-12
- Subcellular Location
- Secreted.
- Protein Name
- Kallikrein-12
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.095 0.191 0.389 0.697 1.274 1.745 2.024 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.095 0.191 0.389 0.697 1.274 1.745 2.024 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.095 0.191 0.389 0.697 1.274 1.745 2.024 O.D. 0.019 0.095 0.191 0.389 0.697 1.274 1.745 2.024
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
