Human KLK13/Kallikrein-13 ELISA Kit PicoKine®

About this product

Human KLK13/Klk L4 ELISA Kit PicoKine® (96 Tests) . Quantitate Human KLK13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1168

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Kallikrein-13 is a protein that in humans is encoded by the KLK13 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. By genomic sequence analysis, KLK13 gene is mapped in a 300-kb region on chromosome 19q13.3-q13.4. It has been shown that recombinant hK13 produced in yeast can cleave synthetic peptides after the arginine residue and some extracellular matrix components. However, its exact physiological substrates and functions remain obscure. Despite the lack of knowledge on the physiological function of hK13, several studies have demonstrated that hK13 is implicated with cancer of the breast and ovary and it can serve as a favorable prognostic biomarker for these malignancies.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 212 215 228 224 219 6.49 2.9% Sample 2 2051 2123 1928 1953 2013 78.04 3.8% Sample 3 5005 5101 4716 5021 4960 145.91 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 212 215 228 224 219 6.49 2.9% Sample 1 212 215 228 224 219 6.49 2.9% Sample 2 2051 2123 1928 1953 2013 78.04 3.8% Sample 2 2051 2123 1928 1953 2013 78.04 3.8% Sample 3 5005 5101 4716 5021 4960 145.91 2.9% Sample 3 5005 5101 4716 5021 4960 145.91 2.9%

Synonyms

Kallikrein-13;3.4.21.-; Kallikrein-like protein 4; KLK-L4; KLK13; KLKL4

Gene Name

KLK13

Gene ID

26085

UniProt

Q9UKR3

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G17-Q277

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in prostate, breast, testis and salivary gland.

Applications

ELISA

Field of Research

Extracellular Matrix, Invasion/Microenvironment, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 212 2051 5005 201 2148 5470 Standard deviation 16.74 159.97 227.92 18.09 186.87 333.67 CV (%) 7.9% 7.8% 4.5% 9% 8.7% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 212 2051 5005 201 2148 5470 Standard deviation 16.74 159.97 227.92 18.09 186.87 333.67 CV (%) 7.9% 7.8% 4.5% 9% 8.7% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 212 2051 5005 201 2148 5470 Mean (pg/ml) 212 2051 5005 201 2148 5470 Standard deviation 16.74 159.97 227.92 18.09 186.87 333.67 Standard deviation 16.74 159.97 227.92 18.09 186.87 333.67 CV (%) 7.9% 7.8% 4.5% 9% 8.7% 6.1% CV (%) 7.9% 7.8% 4.5% 9% 8.7% 6.1%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

30570 MW

Specificity

Natural and recombinant human KLK13

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Kallikrein-13

Subcellular Location

Secreted .

Protein Name

Kallikrein-13

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.075 0.104 0.204 0.464 0.838 1.386 2.028 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.075 0.104 0.204 0.464 0.838 1.386 2.028 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.075 0.104 0.204 0.464 0.838 1.386 2.028 O.D. 0.049 0.075 0.104 0.204 0.464 0.838 1.386 2.028

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.