- Catalog number
- EK1395
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 196 173 198 192 189 9.9 5.2% Sample 2 1010 1076 1107 932 1031 67.16 6.5% Sample 3 4381 3736 4082 3649 3962 291.12 7.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 196 173 198 192 189 9.9 5.2% Sample 1 196 173 198 192 189 9.9 5.2% Sample 2 1010 1076 1107 932 1031 67.16 6.5% Sample 2 1010 1076 1107 932 1031 67.16 6.5% Sample 3 4381 3736 4082 3649 3962 291.12 7.3% Sample 3 4381 3736 4082 3649 3962 291.12 7.3%
- Synonyms
- Proepiregulin; Epiregulin; EPR; Ereg
- Gene Name
- Ereg
- Gene ID
- 13874
- UniProt
- Q61521
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: V53-L101
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Growth Factors/Hormones, Signal Transduction, Tumor Biomarkers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 125 pg/mL - 8,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 196 1010 4381 179 1069 4367 Standard deviation 11.76 66.5%5 2213.961 13.96 73.76 231.45 CV (%) 6% 6.5% 5.2% 7.8% 6.9% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 196 1010 4381 179 1069 4367 Standard deviation 11.76 66.5%5 2213.961 13.96 73.76 231.45 CV (%) 6% 6.5% 5.2% 7.8% 6.9% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 196 1010 4381 179 1069 4367 Mean (pg/ml) 196 1010 4381 179 1069 4367 Standard deviation 11.76 66.5%5 2213.961 13.96 73.76 231.45 Standard deviation 11.76 66.5%5 2213.961 13.96 73.76 231.45 CV (%) 6% 6.5% 5.2% 7.8% 6.9% 5.3% CV (%) 6% 6.5% 5.2% 7.8% 6.9% 5.3%
- Sensitivity
- <10 pg/mL
- Function
- Ligand of the EGF receptor/EGFR and ERBB4. Stimulates EGFR and ERBB4 tyrosine phosphorylation (By similarity) . Contributes to inflammation, wound healing, tissue repair, and oocyte maturation by regulating angiogenesis and vascular remodeling and by stimulating cell proliferation (PubMed:24631357) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 18474 MW
- Specificity
- Natural and recombinant mouse Epiregulin
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Proepiregulin
- Subcellular Location
- Epiregulin: Secreted, extracellular space .
- Protein Name
- Proepiregulin
- Sample Data
- Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.005 0.019 0.047 0.085 0.178 0.41 0.879 1.813 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.005 0.019 0.047 0.085 0.178 0.41 0.879 1.813 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.005 0.019 0.047 0.085 0.178 0.41 0.879 1.813 O.D. 0.005 0.019 0.047 0.085 0.178 0.41 0.879 1.813
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
