- Catalog number
- EK1402
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cluster of differentiation 97 is a protein also known as BL-Ac [F2] encoded by the ADGRE5 gene. The CD97 gene is mapped to 19p13.2-p13.12 by fluorescence in situ hybridization. In the immune system, CD97 is known as a critical mediator of host defense. Upon lymphoid, myeloid cells and neutrophil activation, CD97 is upregulated to promote adhesion and migration to sites of inflammation. Moreover, it has been shown that CD97 regulates granulocyte homeostasis. CD97 is expressed at the sarcoplasmic reticulum and the peripheral sarcolemma in skeletal muscle. However, lack of CD97 only affects the structure of the sarcoplasmic reticulum, but not the function of skeletal muscle.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 192 169 180 190 182 9.14 5% Sample 2 1210 1172 1113 1111 1151 41.72 3.6% Sample 3 5765 6054 5419 5224 5615 318.79 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 192 169 180 190 182 9.14 5% Sample 1 192 169 180 190 182 9.14 5% Sample 2 1210 1172 1113 1111 1151 41.72 3.6% Sample 2 1210 1172 1113 1111 1151 41.72 3.6% Sample 3 5765 6054 5419 5224 5615 318.79 5.6% Sample 3 5765 6054 5419 5224 5615 318.79 5.6%
- Synonyms
- CD97 antigen; Leukocyte antigen CD97; CD97; CD97 antigen subunit alpha; CD97 antigen subunit beta; CD97
- Gene Name
- ADGRE5
- Gene ID
- 976
- UniProt
- P48960
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q21-Q398
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompanied by detectable levels of soluble CD97 in the synovial fluid.
- Applications
- ELISA
- Field of Research
- Cell Type Markers, G Protein Signaling, Hematopoietic Progenitors, Immunology, More Gpcr, Neuroscience, Neurotransmission, Receptors / Channels, Signal Transduction, Signaling Pathway, Stem Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 125 pg/mL - 8,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 192 1210 5765 193 1108 6274 Standard deviation 12.09 62.92 253.66 13.12 77.56 288.6 CV (%) 7% 5.2% 4.4% 6.8% 7% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 192 1210 5765 193 1108 6274 Standard deviation 12.09 62.92 253.66 13.12 77.56 288.6 CV (%) 7% 5.2% 4.4% 6.8% 7% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 192 1210 5765 193 1108 6274 Mean (pg/ml) 192 1210 5765 193 1108 6274 Standard deviation 12.09 62.92 253.66 13.12 77.56 288.6 Standard deviation 12.09 62.92 253.66 13.12 77.56 288.6 CV (%) 7% 5.2% 4.4% 6.8% 7% 4.6% CV (%) 7% 5.2% 4.4% 6.8% 7% 4.6%
- Sensitivity
- <10 pg/mL
- Function
- Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 91869 MW
- Specificity
- Natural and recombinant human CD97
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- CD97 antigen
- Subcellular Location
- Cell membrane; Multi-pass membrane protein.
- Protein Name
- CD97 antigen
- Sample Data
- Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.063 0.095 0.133 0.216 0.358 0.72 1.405 2.098 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.063 0.095 0.133 0.216 0.358 0.72 1.405 2.098 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.063 0.095 0.133 0.216 0.358 0.72 1.405 2.098 O.D. 0.063 0.095 0.133 0.216 0.358 0.72 1.405 2.098
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
