- Catalog number
- EK1406
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Catechol-O-methyltransferase (COMT) is one of several enzymes that degrade catecholamines such as dopamine, epinephrine, and norepinephrine. In humans, catechol-O-methyltransferase protein is encoded by the COMT gene. As the regulation of catecholamines is impaired in a number of medical conditions, several pharmaceutical drugs target COMT to alter its activity and therefore the availability of catecholamines. In the brain, COMT-dependent dopamine degradation is of particular importance in brain regions with low expression of the presynaptic dopamine transporter (DAT), such as the prefrontal cortex. This process is supposed to take place in postsynaptic neurons, as, in general, COMT is located intracellularly in the central nervous system (CNS) .
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 71 72 67 62 68 3.93 5.7% Sample 2 245 245 279 270 259 15.08 5.8% Sample 3 923 1061 1051 966 1000 57.89 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 71 72 67 62 68 3.93 5.7% Sample 1 71 72 67 62 68 3.93 5.7% Sample 2 245 245 279 270 259 15.08 5.8% Sample 2 245 245 279 270 259 15.08 5.8% Sample 3 923 1061 1051 966 1000 57.89 5.7% Sample 3 923 1061 1051 966 1000 57.89 5.7%
- Synonyms
- Catechol O-methyltransferase;2.1.1.6; COMT
- Gene Name
- COMT
- Gene ID
- 1312
- UniProt
- P21964
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: G52-P271
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Brain, liver, placenta, lymphocytes and erythrocytes.
- Applications
- ELISA
- Field of Research
- Cancer, Drug Metabolism, Metabolic Signaling Pathways, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Pathways and Processes, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 71 245 923 65 220 946 Standard deviation 4.97 16.17 61.84 8.43 16.5 79.46 CV (%) 7% 6.6% 6.7% 7.9% 7.5% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 71 245 923 65 220 946 Standard deviation 4.97 16.17 61.84 8.43 16.5 79.46 CV (%) 7% 6.6% 6.7% 7.9% 7.5% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 71 245 923 65 220 946 Mean (pg/ml) 71 245 923 65 220 946 Standard deviation 4.97 16.17 61.84 8.43 16.5 79.46 Standard deviation 4.97 16.17 61.84 8.43 16.5 79.46 CV (%) 7% 6.6% 6.7% 7.9% 7.5% 8.4% CV (%) 7% 6.6% 6.7% 7.9% 7.5% 8.4%
- Sensitivity
- <10 pg/mL
- Function
- Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 30037 MW
- Specificity
- Natural and recombinant human COMT
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Catechol O-methyltransferase
- Subcellular Location
- Isoform Soluble: Cytoplasm.
- Protein Name
- Catechol O-methyltransferase
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.037 0.096 0.153 0.265 0.487 0.882 1.678 2.837 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.037 0.096 0.153 0.265 0.487 0.882 1.678 2.837 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.037 0.096 0.153 0.265 0.487 0.882 1.678 2.837 O.D. 0.037 0.096 0.153 0.265 0.487 0.882 1.678 2.837
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
