Mouse OSM/Oncostatin M ELISA Kit PicoKine®

About this product

Mouse OSM/Oncostatin M ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Osm in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0479

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

OSM (Oncostatin M) is a protein that in humans is encoded by the OSM gene. OSM is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines. The OSM gene is mapped on 22q12.2. Of these cytokines it most closely resembles leukemia inhibitory factor (LIF) in both structure and function. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 57 59 53 57 2.95 5.1% Sample 2 260 248 220 250 244 14.85 6% Sample 3 822 851 792 912 844 44.33 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 57 59 53 57 2.95 5.1% Sample 1 61 57 59 53 57 2.95 5.1% Sample 2 260 248 220 250 244 14.85 6% Sample 2 260 248 220 250 244 14.85 6% Sample 3 822 851 792 912 844 44.33 5.2% Sample 3 822 851 792 912 844 44.33 5.2%

Synonyms

Oncostatin-M; OSM; Osm

Gene Name

Osm

Gene ID

18413

UniProt

P53347

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A24-R206

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cytokines, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Interleukins, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 260 822 59 280 850 Standard deviation 4.51 16.64 36.16 5.31 22.4 52.7 CV (%) 7.4% 6.4% 4.4% 9% 8% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 260 822 59 280 850 Standard deviation 4.51 16.64 36.16 5.31 22.4 52.7 CV (%) 7.4% 6.4% 4.4% 9% 8% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 61 260 822 59 280 850 Mean (pg/ml) 61 260 822 59 280 850 Standard deviation 4.51 16.64 36.16 5.31 22.4 52.7 Standard deviation 4.51 16.64 36.16 5.31 22.4 52.7 CV (%) 7.4% 6.4% 4.4% 9% 8% 6.2% CV (%) 7.4% 6.4% 4.4% 9% 8% 6.2%

Sensitivity

<10 pg/mL

Function

Growth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells (By similarity) . Uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST) . Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration (By similarity) . .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

30114 MW

Specificity

Natural and recombinant mouse Oncostatin M

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Oncostatin-M

Subcellular Location

Secreted.

Protein Name

Oncostatin-M

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.029 0.098 0.157 0.26 0.474 0.924 1.63 2.807 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.029 0.098 0.157 0.26 0.474 0.924 1.63 2.807 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.029 0.098 0.157 0.26 0.474 0.924 1.63 2.807 O.D. 0.029 0.098 0.157 0.26 0.474 0.924 1.63 2.807

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.