- Catalog number
- EK0748
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Decoy receptor 3 (Dcr3), also known as tumor necrosis factor receptor superfamily member 6B (TNFRSF6B), TR6 and M68, is a soluble protein of the tumor necrosis factor receptor superfamily which inhibits Fas ligand-inducedapoptosis. This gene belongs to the tumor necrosis factor receptor superfamily. It acts as a decoy receptor that competes with death receptors for ligand binding. The encoded protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death and T cell activation as well as to induce angiogenesis via neutralization of TL1A. Overexpression of this gene has been noted in various tumors e.g. gastrointestinal tract tumors, and it is located in a gene-rich cluster on chromosome 20, with other potentially tumor-related genes.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 246 272 280 267 12.83 4.8% Sample 2 1644 1634 1754 1881 1728 99.97 5.7% Sample 3 5011 5645 5121 5650 5356 293.34 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 246 272 280 267 12.83 4.8% Sample 1 272 246 272 280 267 12.83 4.8% Sample 2 1644 1634 1754 1881 1728 99.97 5.7% Sample 2 1644 1634 1754 1881 1728 99.97 5.7% Sample 3 5011 5645 5121 5650 5356 293.34 5.4% Sample 3 5011 5645 5121 5650 5356 293.34 5.4%
- Synonyms
- Tumor necrosis factor receptor superfamily member 6B; Decoy receptor 3; DcR3; Decoy receptor for Fas ligand; M68; TNFRSF6B; DCR3, TR6; UNQ186/PRO212
- Gene Name
- TNFRSF6B
- Gene ID
- 8771
- UniProt
- O95407
- Reactivity
- Human
- Cross Reactivity
- No cross reactivity with other proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: V24-H300
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells.
- Applications
- ELISA
- Field of Research
- Apoptosis, Cancer, Cell Biology, Cell Death, Cytokines, Immunology, Innate Immunity, Receptors
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1644 5011 272 1715 5192 Standard deviation 12.24 1441.324 330.72 14.96 145.77 441.32 CV (%) 4.5% 7.6% 6.6% 5.5% 8.5% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1644 5011 272 1715 5192 Standard deviation 12.24 1441.324 330.72 14.96 145.77 441.32 CV (%) 4.5% 7.6% 6.6% 5.5% 8.5% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 272 1644 5011 272 1715 5192 Mean (pg/ml) 272 1644 5011 272 1715 5192 Standard deviation 12.24 1441.324 330.72 14.96 145.77 441.32 Standard deviation 12.24 1441.324 330.72 14.96 145.77 441.32 CV (%) 4.5% 7.6% 6.6% 5.5% 8.5% 8.5% CV (%) 4.5% 7.6% 6.6% 5.5% 8.5% 8.5%
- Sensitivity
- <10 pg/mL
- Function
- Decoy receptor that can neutralize the cytotoxic ligands TNFS14/LIGHT, TNFSF15 and TNFSF6/FASL. Protects against apoptosis. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 32680 MW
- Specificity
- Natural and recombinant human DCR3
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Tumor necrosis factor receptor superfamily member 6B
- Subcellular Location
- Secreted.
- Protein Name
- Tumor necrosis factor receptor superfamily member 6B
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.047 0.11 0.174 0.312 0.577 1.014 1.808 2.411 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.047 0.11 0.174 0.312 0.577 1.014 1.808 2.411 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.047 0.11 0.174 0.312 0.577 1.014 1.808 2.411 O.D. 0.047 0.11 0.174 0.312 0.577 1.014 1.808 2.411
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
