- Catalog number
- EK1184
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 116 132 124 119 122 6.05 4.9% Sample 2 727 733 850 715 756 54.51 7.2% Sample 3 2784 2655 2448 2326 2553 177.7 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 116 132 124 119 122 6.05 4.9% Sample 1 116 132 124 119 122 6.05 4.9% Sample 2 727 733 850 715 756 54.51 7.2% Sample 2 727 733 850 715 756 54.51 7.2% Sample 3 2784 2655 2448 2326 2553 177.7 6.9% Sample 3 2784 2655 2448 2326 2553 177.7 6.9%
- Synonyms
- CD166 antigen; Activated leukocyte cell adhesion molecule ; BEN ; Protein DM-GRASP ; CD166; Alcam
- Gene Name
- Alcam
- Gene ID
- 11658
- UniProt
- Q61490
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: W28-K527
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Detected on brain motor neurons, in differentiating retinal ganglion cells and in adult retina (PubMed:15345243) . Detected on leukocytes and on lymphatic endothelial cells (PubMed:23169771) . Detected in spleen B cells and T-cells (at protein level) (PubMed:9209500) . Detected in adult brain and embryonic spinal cord (PubMed:15345243) . Expressed at high levels in the brain, and lung, and at lower levels in the liver, and the kidney, as well as by activated leukocytes (PubMed:9209500) . .
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, B Cells, Immunology, T Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 78 pg/mL - 5,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 116 727 2784 112 706 2677 Standard deviation 8.58 29.8 219.93 7.4%8 32.47 211.48 CV (%) 7.4% 4.1% 7.9% 7.4% 4.6% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 116 727 2784 112 706 2677 Standard deviation 8.58 29.8 219.93 7.4%8 32.47 211.48 CV (%) 7.4% 4.1% 7.9% 7.4% 4.6% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 116 727 2784 112 706 2677 Mean (pg/ml) 116 727 2784 112 706 2677 Standard deviation 8.58 29.8 219.93 7.4%8 32.47 211.48 Standard deviation 8.58 29.8 219.93 7.4%8 32.47 211.48 CV (%) 7.4% 4.1% 7.9% 7.4% 4.6% 7.9% CV (%) 7.4% 4.1% 7.9% 7.4% 4.6% 7.9%
- Sensitivity
- <10 pg/mL
- Function
- Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6 (By similarity) . Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (By similarity) . Mediates homotypic interactions with cells that express ALCAM (PubMed:24740813) . Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:23169771) . Required for normal organization of the lymph vessel network (PubMed:23169771) . Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813) . Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow (PubMed:25730656) . Promotes in vitro osteoblast proliferation and differentiation (PubMed:25730656) . Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM (By similarity) . Mediates outgrowth and pathfinding for retinal ganglion cell axons (PubMed:15345243) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 65092 MW
- Specificity
- Natural and recombinant mouse ALCAM
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- CD166 antigen
- Subcellular Location
- Cell membrane ; Single-pass type I membrane protein . Cell projection, axon . Cell projection, dendrite . Detected at the immunological synapse, i.e, at the contact zone between antigen-presenting dendritic cells and T-cells. Colocalizes with CD6 and the TCR/CD3 complex at the immunological synapse. .
- Protein Name
- CD166 antigen
- Sample Data
- Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.028 0.099 0.147 0.261 0.462 0.834 1.387 2.079 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.028 0.099 0.147 0.261 0.462 0.834 1.387 2.079 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.028 0.099 0.147 0.261 0.462 0.834 1.387 2.079 O.D. 0.028 0.099 0.147 0.261 0.462 0.834 1.387 2.079
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
