- Catalog number
- EK1162
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Granzyme A is a protein that in humans is encoded by the GZMA gene. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface nonself antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. GZMA induces caspase-independent apoptosis in a characteristic manner, except it causes a distinctive form of DNA damage: single-stranded DNA nicking. A target of GZMA is the SET complex, including HMGB2 and ANP32A.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 38 38 38 38 0.86 2.2% Sample 2 218 224 234 218 223 6.53 2.9% Sample 3 455 435 399 422 427 20.33 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 38 38 38 38 0.86 2.2% Sample 1 40 38 38 38 38 0.86 2.2% Sample 2 218 224 234 218 223 6.53 2.9% Sample 2 218 224 234 218 223 6.53 2.9% Sample 3 455 435 399 422 427 20.33 4.7% Sample 3 455 435 399 422 427 20.33 4.7%
- Synonyms
- Granzyme A;3.4.21.78; CTL tryptase; Cytotoxic T-lymphocyte proteinase 1; Fragmentin-1; Granzyme-1; Hanukkah factor; H factor; HF; GZMA; CTLA3, HFSP
- Gene Name
- GZMA
- Gene ID
- 3001
- UniProt
- P12544
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: I29-V262
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Apoptosis, Cell Biology, Immunology, Innate Immunity, NK Cells, T Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 218 455 42 207 410 Standard deviation 2 13.73 27.3 2.73 17.9%5 25.42 CV (%) 5% 7.9%% 6% 6.5% 7.9% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 218 455 42 207 410 Standard deviation 2 13.73 27.3 2.73 17.9%5 25.42 CV (%) 5% 7.9%% 6% 6.5% 7.9% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 40 218 455 42 207 410 Mean (pg/ml) 40 218 455 42 207 410 Standard deviation 2 13.73 27.3 2.73 17.9%5 25.42 Standard deviation 2 13.73 27.3 2.73 17.9%5 25.42 CV (%) 5% 7.9%% 6% 6.5% 7.9% 6.2% CV (%) 5% 7.9%% 6% 6.5% 7.9% 6.2%
- Sensitivity
- <10 pg/mL
- Function
- Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent cell death with morphological features of apoptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 28999 MW
- Specificity
- Natural and recombinant human Granzyme A
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Granzyme A
- Subcellular Location
- Isoform alpha: Secreted. Cytoplasmic granule.
- Protein Name
- Granzyme A
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.058 0.188 0.282 0.409 0.716 1.056 1.533 1.914 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.058 0.188 0.282 0.409 0.716 1.056 1.533 1.914 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.058 0.188 0.282 0.409 0.716 1.056 1.533 1.914 O.D. 0.058 0.188 0.282 0.409 0.716 1.056 1.533 1.914
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
