- Catalog number
- EK1240
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Galectin-3-binding protein is a protein that in humans is encoded by the LGALS3BP gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell–cell and cell–matrix interactions. Using fluorescence in–situ hybridization, the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds to galectin 1.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 312 367 361 366 351 22.91 6.5% Sample 2 1090 1067 1143 1041 1085 37.57 3.4% Sample 3 4158 3836 4350 4159 4125 184.65 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 312 367 361 366 351 22.91 6.5% Sample 1 312 367 361 366 351 22.91 6.5% Sample 2 1090 1067 1143 1041 1085 37.57 3.4% Sample 2 1090 1067 1143 1041 1085 37.57 3.4% Sample 3 4158 3836 4350 4159 4125 184.65 4.4% Sample 3 4158 3836 4350 4159 4125 184.65 4.4%
- Synonyms
- Galectin-3-binding protein; Basement membrane autoantigen p105; Lectin galactoside-binding soluble 3-binding protein; Mac-2-binding protein; MAC2BP; Mac-2 BP; Tumor-associated antigen 90K; LGALS3BP; M2BP
- Gene Name
- LGALS3BP
- Gene ID
- 3959
- UniProt
- Q08380
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: V19-D585
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Monoclonal antibody from mouse
- Tissue Specificity
- Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts. .
- Applications
- ELISA
- Field of Research
- Cancer, Cell Cycle, Cell Division, Oncoproteins, Tumor Biomarkers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 312 1090 4158 326 1154 3768 Standard deviation 18.72 63.22 282.74 20.86 84.24 271.29 CV (%) 6% 5.8% 6.8% 6.4% 7.3% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 312 1090 4158 326 1154 3768 Standard deviation 18.72 63.22 282.74 20.86 84.24 271.29 CV (%) 6% 5.8% 6.8% 6.4% 7.3% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 312 1090 4158 326 1154 3768 Mean (pg/ml) 312 1090 4158 326 1154 3768 Standard deviation 18.72 63.22 282.74 20.86 84.24 271.29 Standard deviation 18.72 63.22 282.74 20.86 84.24 271.29 CV (%) 6% 5.8% 6.8% 6.4% 7.3% 7.2% CV (%) 6% 5.8% 6.8% 6.4% 7.3% 7.2%
- Sensitivity
- <10 pg/mL
- Function
- Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 65331 MW
- Specificity
- Natural and recombinant human Galectin-3BP
- Anticoagulant
- Heparin
- Gene Name Synonym
- Galectin-3-binding protein
- Subcellular Location
- Secreted . Secreted, extracellular space, extracellular matrix .
- Protein Name
- Galectin-3-binding protein
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.125 0.189 0.309 0.574 0.956 1.437 2.064 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.125 0.189 0.309 0.574 0.956 1.437 2.064 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.125 0.189 0.309 0.574 0.956 1.437 2.064 O.D. 0.055 0.125 0.189 0.309 0.574 0.956 1.437 2.064
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
