- Catalog number
- EK0676
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cathepsin L is an important lysosomal endopeptidase enzyme which is involved in the initiation of protein degradation. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. The gene is mapped to 9q21-q22. It is a member of the Peptidase C1 family, which play an important role in diverse processes including normal lysosome mediated protein turnover, antigen and proprotein processing, and apoptosis. Cathepsin L has been reported in many organisms including fish, birds and mammals.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 204 202 210 182 199 10.52 5.2% Sample 2 726 748 779 754 751 18.87 2.5% Sample 3 1367 1597 1607 1555 1531 96.95 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 204 202 210 182 199 10.52 5.2% Sample 1 204 202 210 182 199 10.52 5.2% Sample 2 726 748 779 754 751 18.87 2.5% Sample 2 726 748 779 754 751 18.87 2.5% Sample 3 1367 1597 1607 1555 1531 96.95 6.3% Sample 3 1367 1597 1607 1555 1531 96.95 6.3%
- Synonyms
- Cathepsin L1;3.4.22.15; Cathepsin L; Major excreted protein; MEP; Cathepsin L1 heavy chain; Cathepsin L1 light chain; CTSL; CTSL1
- Gene Name
- CTSL
- Gene ID
- 1514
- UniProt
- P07711
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E113-V333
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cell Adhesion Proteins, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Membrane Proteins, Neuroscience, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 204 726 1367 205 706 1305 Standard deviation 10.2 29.76 66.98 11.07 36.71 82.21 CV (%) 5% 4.1% 4.9% 5.4% 5.2% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 204 726 1367 205 706 1305 Standard deviation 10.2 29.76 66.98 11.07 36.71 82.21 CV (%) 5% 4.1% 4.9% 5.4% 5.2% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 204 726 1367 205 706 1305 Mean (pg/ml) 204 726 1367 205 706 1305 Standard deviation 10.2 29.76 66.98 11.07 36.71 82.21 Standard deviation 10.2 29.76 66.98 11.07 36.71 82.21 CV (%) 5% 4.1% 4.9% 5.4% 5.2% 6.3% CV (%) 5% 4.1% 4.9% 5.4% 5.2% 6.3%
- Sensitivity
- <10 pg/mL
- Function
- Important for the overall degradation of proteins in lysosomes.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 37564 MW
- Specificity
- Natural and recombinant human Cathepsin L
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Cathepsin L1
- Subcellular Location
- Lysosome.
- Protein Name
- Cathepsin L1
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.102 0.151 0.252 0.453 0.783 1.331 1.926 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.102 0.151 0.252 0.453 0.783 1.331 1.926 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.102 0.151 0.252 0.453 0.783 1.331 1.926 O.D. 0.034 0.102 0.151 0.252 0.453 0.783 1.331 1.926
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
