- Catalog number
- EK0766
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The granulocyte colony-stimulating factor receptor (G-CSF-R), also known as CD114 (Cluster of Differentiation 114), is a protein that in humans is encoded by the CSF3R gene. It is mapped to 1p35-p34.3. G-CSF-R is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF) . And the G-CSF-R is a transmembrane receptor that consists of an extracellular ligand-binding portion, a transmembrane domain, and the cytoplasmic portion that is responsible for signal transduction. In addition, GCSF-R ligand-binding is associated with dimerization of the receptor and signal transduction through proteins including Jak, Lyn, STAT, andErk1/2.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 72 77 84 86 79 5.58 7% Sample 2 222 224 255 245 236 13.97 5.9% Sample 3 823 879 872 734 827 57.86 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 72 77 84 86 79 5.58 7% Sample 1 72 77 84 86 79 5.58 7% Sample 2 222 224 255 245 236 13.97 5.9% Sample 2 222 224 255 245 236 13.97 5.9% Sample 3 823 879 872 734 827 57.86 6.9% Sample 3 823 879 872 734 827 57.86 6.9%
- Synonyms
- Granulocyte colony-stimulating factor receptor; G-CSF receptor; G-CSF-R; CD114; CSF3R; GCSFR
- Gene Name
- CSF3R
- Gene ID
- 1441
- UniProt
- Q99062
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E25-P621
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- One or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U-937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U-937 cells. Isoform GCSFR-3 is highly expressed in placenta.
- Applications
- ELISA
- Field of Research
- Cancer, Cytokines, Hematopoietic Progenitors, Immunology, Innate Immunity, Oncoproteins, Oncoproteins/Suppressors, Stem Cells, Surface Molecules
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 72 222 823 69 240 838 Standard deviation 5.54 9.99 59.25 6.27 18.12 67.87 CV (%) 7.7% 4.5% 7.2% 9.1% 6.3% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 72 222 823 69 240 838 Standard deviation 5.54 9.99 59.25 6.27 18.12 67.87 CV (%) 7.7% 4.5% 7.2% 9.1% 6.3% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 72 222 823 69 240 838 Mean (pg/ml) 72 222 823 69 240 838 Standard deviation 5.54 9.99 59.25 6.27 18.12 67.87 Standard deviation 5.54 9.99 59.25 6.27 18.12 67.87 CV (%) 7.7% 4.5% 7.2% 9.1% 6.3% 8.1% CV (%) 7.7% 4.5% 7.2% 9.1% 6.3% 8.1%
- Sensitivity
- <10 pg/mL
- Function
- Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 92156 MW
- Specificity
- Natural and recombinant human CSF3R
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Granulocyte colony-stimulating factor receptor
- Subcellular Location
- Isoform 2: Secreted .
- Protein Name
- Granulocyte colony-stimulating factor receptor
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.238 0.332 0.512 0.733 1.185 1.722 2.582 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.238 0.332 0.512 0.733 1.185 1.722 2.582 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.238 0.332 0.512 0.733 1.185 1.722 2.582 O.D. 0.028 0.238 0.332 0.512 0.733 1.185 1.722 2.582
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
