Rat MMP-8/Neutrophil collagenase ELISA Kit PicoKine®

About this product

Rat MMP-8 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Mmp8 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1248

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Matrix metalloproteinase 8 (MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase (HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 238 216 228 210 223 10.81 4.8% Sample 2 1865 1657 1883 1979 1846 117.4 6.3% Sample 3 3841 4372 4478 4486 4294 265.52 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 238 216 228 210 223 10.81 4.8% Sample 1 238 216 228 210 223 10.81 4.8% Sample 2 1865 1657 1883 1979 1846 117.4 6.3% Sample 2 1865 1657 1883 1979 1846 117.4 6.3% Sample 3 3841 4372 4478 4486 4294 265.52 6.1% Sample 3 3841 4372 4478 4486 4294 265.52 6.1%

Synonyms

Neutrophil collagenase;3.4.24.34; Matrix metalloproteinase-8; MMP-8; Mmp8

Gene Name

Mmp8

Gene ID

63849

UniProt

O88766

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L21-P466

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Can degrade fibrillar type I, II, and III collagens.

Applications

ELISA

Field of Research

Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction, Tumor Biomarkers, Vascular Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 238 1865 3841 261 1840 3780 Standard deviation 18.08 91.38 261.18 21.92 99.36 325.08 CV (%) 7.6% 4.9% 6.8% 8.4% 5.4% 8.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 238 1865 3841 261 1840 3780 Standard deviation 18.08 91.38 261.18 21.92 99.36 325.08 CV (%) 7.6% 4.9% 6.8% 8.4% 5.4% 8.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 238 1865 3841 261 1840 3780 Mean (pg/ml) 238 1865 3841 261 1840 3780 Standard deviation 18.08 91.38 261.18 21.92 99.36 325.08 Standard deviation 18.08 91.38 261.18 21.92 99.36 325.08 CV (%) 7.6% 4.9% 6.8% 8.4% 5.4% 8.6% CV (%) 7.6% 4.9% 6.8% 8.4% 5.4% 8.6%

Sensitivity

<10 pg/mL

Function

Can degrade fibrillar type I, II, and III collagens.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

53277 MW

Specificity

Natural and recombinant rat MMP-8

Anticoagulant

Heparin

Gene Name Synonym

Neutrophil collagenase

Subcellular Location

Cytoplasmic granule. Secreted, extracellular space, extracellular matrix . Stored in intracellular granules.

Sequence Similarities

Belongs to the peptidase M10A family.

Protein Name

Neutrophil collagenase

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.074 0.145 0.297 0.57 1.038 1.58 2.05 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.074 0.145 0.297 0.57 1.038 1.58 2.05 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.074 0.145 0.297 0.57 1.038 1.58 2.05 O.D. 0.011 0.074 0.145 0.297 0.57 1.038 1.58 2.05

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.