- Catalog number
- EK0755
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. Also it is involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since it is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 35 32 35 35 2.12 6% Sample 2 143 161 140 157 150 8.92 5.9% Sample 3 444 418 452 413 431 16.58 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 35 32 35 35 2.12 6% Sample 1 38 35 32 35 35 2.12 6% Sample 2 143 161 140 157 150 8.92 5.9% Sample 2 143 161 140 157 150 8.92 5.9% Sample 3 444 418 452 413 431 16.58 3.8% Sample 3 444 418 452 413 431 16.58 3.8%
- Synonyms
- Epithelial cell adhesion molecule; Ep-CAM; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; EGP314; hEGP314; KS 1/4 antigen; KSA; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; CD326; EPCAM; GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1
- Gene Name
- EPCAM
- Gene ID
- 4072
- UniProt
- P16422
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q24-K265
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC) . Levels rapidly diminish as soon as ESC's differentiate (at protein levels) . Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .
- Applications
- ELISA
- Field of Research
- Cell Type Markers, Tags & Cell Markers, Tumor Associated
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 143 444 35 150 444 Standard deviation 1.63 6 33.74 1.82 7.5 37.29 CV (%) 4.3% 4.2% 7.6% 5.2% 5% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 143 444 35 150 444 Standard deviation 1.63 6 33.74 1.82 7.5 37.29 CV (%) 4.3% 4.2% 7.6% 5.2% 5% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 38 143 444 35 150 444 Mean (pg/ml) 38 143 444 35 150 444 Standard deviation 1.63 6 33.74 1.82 7.5 37.29 Standard deviation 1.63 6 33.74 1.82 7.5 37.29 CV (%) 4.3% 4.2% 7.6% 5.2% 5% 8.4% CV (%) 4.3% 4.2% 7.6% 5.2% 5% 8.4%
- Sensitivity
- <10 pg/mL
- Function
- May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 34932 MW
- Specificity
- Natural and recombinant human EPCAM
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Epithelial cell adhesion molecule
- Subcellular Location
- Lateral cell membrane ; Single-pass type I membrane protein . Cell junction, tight junction . Colocalizes with CLDN7 at the lateral cell membrane and tight junction. .
- Sequence Similarities
- Belongs to the EPCAM family. Contains 1 thyroglobulin type-1 domain.
- Protein Name
- Epithelial cell adhesion molecule
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.064 0.109 0.216 0.401 0.705 1.21 1.932 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.064 0.109 0.216 0.401 0.705 1.21 1.932 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.064 0.109 0.216 0.401 0.705 1.21 1.932 O.D. 0.033 0.064 0.109 0.216 0.401 0.705 1.21 1.932
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
