- Catalog number
- EK1417
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. It is contains 19 exons spanning 50 kb of genomic DNA. In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs) . Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 481 494 514 475 491 14.94 3% Sample 2 1665 1566 1588 1645 1616 40.39 2.4% Sample 3 7855 9238 8886 9256 8808 570.06 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 481 494 514 475 491 14.94 3% Sample 1 481 494 514 475 491 14.94 3% Sample 2 1665 1566 1588 1645 1616 40.39 2.4% Sample 2 1665 1566 1588 1645 1616 40.39 2.4% Sample 3 7855 9238 8886 9256 8808 570.06 6.4% Sample 3 7855 9238 8886 9256 8808 570.06 6.4%
- Synonyms
- CD44 antigen; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I; PGP-I; CD44; Cd44
- Gene Name
- Cd44
- Gene ID
- 25406
- UniProt
- P26051
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q22-E271
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cell Type Markers, Hematopoietic Progenitors, Immunology, Lymphoid, Mesenchymal Stem Cells, Myeloid, Stem Cells, Surface Molecules, T Lymphocytic Lineage, Tumor Biomarkers, Tumor Immunology
- Standard
- 20 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 312 pg/mL - 20,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 481 1665 7855 452 1537 7710 Standard deviation 28.37 129.87 439.88 32.54 136.79 516.57 CV (%) 5.6% 7.8% 5.6% 7.2% 8.9% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 481 1665 7855 452 1537 7710 Standard deviation 28.37 129.87 439.88 32.54 136.79 516.57 CV (%) 5.6% 7.8% 5.6% 7.2% 8.9% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 481 1665 7855 452 1537 7710 Mean (pg/ml) 481 1665 7855 452 1537 7710 Standard deviation 28.37 129.87 439.88 32.54 136.79 516.57 Standard deviation 28.37 129.87 439.88 32.54 136.79 516.57 CV (%) 5.6% 7.8% 5.6% 7.2% 8.9% 6.7% CV (%) 5.6% 7.8% 5.6% 7.2% 8.9% 6.7%
- Sensitivity
- <10 pg/mL
- Function
- Receptor for hyaluronic acid (HA) . Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs) . Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Receptor for LGALS9; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 55946 MW
- Specificity
- Natural and recombinant rat CD44
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- CD44 antigen
- Subcellular Location
- Cell membrane ; Single-pass type I membrane protein . Colocalizes with actin in membrane protrusions at wounding edges. .
- Sequence Similarities
- Contains 1 Link domain.
- Protein Name
- CD44 antigen
- Sample Data
- Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.012 0.093 0.14 0.251 0.443 0.871 1.433 2.321 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.012 0.093 0.14 0.251 0.443 0.871 1.433 2.321 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.012 0.093 0.14 0.251 0.443 0.871 1.433 2.321 O.D. 0.012 0.093 0.14 0.251 0.443 0.871 1.433 2.321
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
