- Catalog number
- EK1421
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD47 (Cluster of Differentiation 47), also known as integrin associated protein (IAP), is a transmembrane protein that in humans is encoded by the CD47 gene. It belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα) . CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. Also CD47 is ubiquitously expressed in human cells and has found to be overexpressed in many different tumor cells.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 193 162 183 189 181 11.94 6.5% Sample 2 888 960 1024 1007 969 52.69 5.4% Sample 3 3988 4066 3941 3907 3975 59.64 1.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 193 162 183 189 181 11.94 6.5% Sample 1 193 162 183 189 181 11.94 6.5% Sample 2 888 960 1024 1007 969 52.69 5.4% Sample 2 888 960 1024 1007 969 52.69 5.4% Sample 3 3988 4066 3941 3907 3975 59.64 1.5% Sample 3 3988 4066 3941 3907 3975 59.64 1.5%
- Synonyms
- Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD47; CD47; MER6
- Gene Name
- CD47
- Gene ID
- 961
- UniProt
- Q08722
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q19-P139
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
- Applications
- ELISA
- Field of Research
- Cancer, Cell Type Markers, Immunology, Tumor Immunology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 125 pg/mL - 8,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 193 888 3988 208 813 3951 Standard deviation 7.72 54.16 183.44 11.02 60.97 256.81 CV (%) 4% 6.1% 4.6% 5.3% 7.5% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 193 888 3988 208 813 3951 Standard deviation 7.72 54.16 183.44 11.02 60.97 256.81 CV (%) 4% 6.1% 4.6% 5.3% 7.5% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 193 888 3988 208 813 3951 Mean (pg/ml) 193 888 3988 208 813 3951 Standard deviation 7.72 54.16 183.44 11.02 60.97 256.81 Standard deviation 7.72 54.16 183.44 11.02 60.97 256.81 CV (%) 4% 6.1% 4.6% 5.3% 7.5% 6.5% CV (%) 4% 6.1% 4.6% 5.3% 7.5% 6.5%
- Sensitivity
- <10 pg/mL
- Function
- Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity) . Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 35214 MW
- Specificity
- Natural and recombinant human CD47
- Gene Name Synonym
- Leukocyte surface antigen CD47
- Subcellular Location
- Cell membrane ; Multi-pass membrane protein .
- Sequence Similarities
- Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Protein Name
- Leukocyte surface antigen CD47
- Sample Data
- Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.033 0.073 0.125 0.213 0.376 0.658 1.12 1.905 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.033 0.073 0.125 0.213 0.376 0.658 1.12 1.905 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.033 0.073 0.125 0.213 0.376 0.658 1.12 1.905 O.D. 0.033 0.073 0.125 0.213 0.376 0.658 1.12 1.905
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
