- Catalog number
- EK1422
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- 5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 230 235 243 260 242 11.37 4.6% Sample 2 1941 1870 1830 1891 1883 40.01 2.1% Sample 3 5361 4741 5455 5425 5245 293.24 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 230 235 243 260 242 11.37 4.6% Sample 1 230 235 243 260 242 11.37 4.6% Sample 2 1941 1870 1830 1891 1883 40.01 2.1% Sample 2 1941 1870 1830 1891 1883 40.01 2.1% Sample 3 5361 4741 5455 5425 5245 293.24 5.5% Sample 3 5361 4741 5455 5425 5245 293.24 5.5%
- Synonyms
- 5'-nucleotidase;5'-NT;3.1.3.5; Ecto-5'-nucleotidase; CD73; NT5E; NT5, NTE
- Gene Name
- NT5E
- Gene ID
- 4907
- UniProt
- Q6NZX3
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: W27-K547
- Capture Antibody
- Polyclonal antibody from sheep
- Detection Antibody
- Polyclonal antibody from sheep
- Applications
- ELISA
- Field of Research
- Associated Proteins, Atherosclerosis, Cardiovascular, Hypoxia, Vascular Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 230 1941 5361 222 1912 4929 Standard deviation 15.41 79.58 387.29 18.64 101.33 359.81 CV (%) 6.7% 4.1% 7.2% 8.4% 5.3% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 230 1941 5361 222 1912 4929 Standard deviation 15.41 79.58 387.29 18.64 101.33 359.81 CV (%) 6.7% 4.1% 7.2% 8.4% 5.3% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 230 1941 5361 222 1912 4929 Mean (pg/ml) 230 1941 5361 222 1912 4929 Standard deviation 15.41 79.58 387.29 18.64 101.33 359.81 Standard deviation 15.41 79.58 387.29 18.64 101.33 359.81 CV (%) 6.7% 4.1% 7.2% 8.4% 5.3% 7.3% CV (%) 6.7% 4.1% 7.2% 8.4% 5.3% 7.3%
- Sensitivity
- <10 pg/mL
- Function
- Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 63368 MW
- Specificity
- Natural and recombinant human CD73
- Anticoagulant
- Heparin or EDTA
- Subcellular Location
- Cell membrane; Lipid-anchor, GPI-anchor.
- Sequence Similarities
- Belongs to the 5'-nucleotidase family.
- Protein Name
- Ecto-5'-nucleotidase
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.099 0.132 0.201 0.348 0.6254 1.135 1.946 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.099 0.132 0.201 0.348 0.6254 1.135 1.946 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.099 0.132 0.201 0.348 0.6254 1.135 1.946 O.D. 0.056 0.099 0.132 0.201 0.348 0.6254 1.135 1.946
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
