- Catalog number
- EK0457
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chemokine (C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta) . Using PCR of human-rodent hybrids, the CCL19 gene was mapped to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 32 28 32 30 1.65 5.5% Sample 2 161 166 173 167 166 4.26 2.5% Sample 3 384 401 402 407 398 8.67 2.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 32 28 32 30 1.65 5.5% Sample 1 30 32 28 32 30 1.65 5.5% Sample 2 161 166 173 167 166 4.26 2.5% Sample 2 161 166 173 167 166 4.26 2.5% Sample 3 384 401 402 407 398 8.67 2.1% Sample 3 384 401 402 407 398 8.67 2.1%
- Synonyms
- C-C motif chemokine 19; Epstein-Barr virus-induced molecule 1 ligand chemokine; EBI1 ligand chemokine; ELC; Small-inducible cytokine A19; Ccl19; Elc, Scya19
- Gene Name
- Ccl19
- Gene ID
- 24047
- UniProt
- O70460
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: G26-S108
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Highly expressed by dendritic cells in mesenteric and peripheral lymph nodes. Significant expression in spleen (T cell zone or periarteriolar lymphatic sheath) and Peyer patches. Low expression in thymus.
- Applications
- ELISA
- Field of Research
- Chemokines, G Protein Signaling, Immunology, Innate Immunity, Macrophage/Inflammation, Signal Transduction, Signaling Pathway
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 161 384 27 175 349 Standard deviation 1.2 8.53 29.56 1.45 9.27 33.5 CV (%) 4% 5.3% 7.7% 5.4% 5.3% 9.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 161 384 27 175 349 Standard deviation 1.2 8.53 29.56 1.45 9.27 33.5 CV (%) 4% 5.3% 7.7% 5.4% 5.3% 9.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 30 161 384 27 175 349 Mean (pg/ml) 30 161 384 27 175 349 Standard deviation 1.2 8.53 29.56 1.45 9.27 33.5 Standard deviation 1.2 8.53 29.56 1.45 9.27 33.5 CV (%) 4% 5.3% 7.7% 5.4% 5.3% 9.6% CV (%) 4% 5.3% 7.7% 5.4% 5.3% 9.6%
- Sensitivity
- <10 pg/mL
- Function
- Strongly chemotactic for naive (L-selectinhi) CD4 T- cells and for CD8 T-cells and weakly attractive for resting B- cells and memory (L-selectinlo) CD4 T-cells. May play a role in promoting encounters between recirculating T-cells and dendritic cells and in the migration of activated B-cells into the T-zone of secondary lymphoid tissues. Binds to chemokine receptor CCR7. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 11911 MW
- Specificity
- Natural and recombinant mouse CCL19
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-C motif chemokine 19
- Subcellular Location
- Secreted.
- Protein Name
- C-C motif chemokine 19
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.005 0.051 0.109 0.198 0.418 0.787 1.297 1.909 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.005 0.051 0.109 0.198 0.418 0.787 1.297 1.909 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.005 0.051 0.109 0.198 0.418 0.787 1.297 1.909 O.D. 0.005 0.051 0.109 0.198 0.418 0.787 1.297 1.909
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
