- Catalog number
- EK1427
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Agouti-related protein, also called Agouti-related peptide (AgRP), is a neuropeptide produced in the brain by the AgRP/NPY neuron. It is only synthesised in NPY-containing cell bodies located in the ventromedial part of the arcuate nucleus in the hypothalamus. In humans, the agouti-related peptide is encoded by the AGRP gene. AgRP is co-expressed with Neuropeptide Y and works by increasing appetite and decreasing metabolism and energy expenditure. It is one of the most potent and long-lasting of appetite stimulators.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 15 18 16 17 16 1.11 6.9% Sample 2 99 100 93 95 96 2.86 2.9% Sample 3 236 218 202 233 222 13.53 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 15 18 16 17 16 1.11 6.9% Sample 1 15 18 16 17 16 1.11 6.9% Sample 2 99 100 93 95 96 2.86 2.9% Sample 2 99 100 93 95 96 2.86 2.9% Sample 3 236 218 202 233 222 13.53 6% Sample 3 236 218 202 233 222 13.53 6%
- Synonyms
- Agouti-related protein; AGRP; AGRT, ART
- Gene Name
- AGRP
- Gene ID
- 181
- UniProt
- O00253
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A21-T132
- Tissue Specificity
- Expressed primarily in the adrenal gland, subthalamic nucleus, and hypothalamus, with a lower level of expression occurring in testis, lung, and kidney.
- Applications
- ELISA
- Field of Research
- Calcium Signaling, Cancer, Cardiovascular, Endocrine System, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Neurology Process, Neuroscience, Obesity, Pathways and Processes, Redox Metabolism, Signal Transduction, Signaling Pathway
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.
- Detection Range
- 7.8 pg/mL - 500 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 15 99 236 13 102 246 Standard deviation 0.63 3.96 10.38 0.68 5.4 12.05 CV (%) 4.2% 4% 4.4% 5.3% 5.3% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 15 99 236 13 102 246 Standard deviation 0.63 3.96 10.38 0.68 5.4 12.05 CV (%) 4.2% 4% 4.4% 5.3% 5.3% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 15 99 236 13 102 246 Mean (pg/ml) 15 99 236 13 102 246 Standard deviation 0.63 3.96 10.38 0.68 5.4 12.05 Standard deviation 0.63 3.96 10.38 0.68 5.4 12.05 CV (%) 4.2% 4% 4.4% 5.3% 5.3% 4.9% CV (%) 4.2% 4% 4.4% 5.3% 5.3% 4.9%
- Sensitivity
- <4 pg/mL
- Function
- Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R (By similarity) . Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 14440 MW
- Specificity
- Natural and recombinant human AGRP
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Agouti-related protein
- Subcellular Location
- Secreted . Golgi apparatus lumen .
- Protein Name
- Agouti-related protein
- Sample Data
- Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.025 0.075 0.119 0.21 0.369 0.749 1.355 2.007 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.025 0.075 0.119 0.21 0.369 0.749 1.355 2.007 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.025 0.075 0.119 0.21 0.369 0.749 1.355 2.007 O.D. 0.025 0.075 0.119 0.21 0.369 0.749 1.355 2.007
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
